Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
96 Cards in this Set
- Front
- Back
component making should be w/in how many hours? |
8 hours |
|
How do you make a platelet concentrate from a whole blood? |
light spin then hard spin |
|
In how many hours should an FFP be made? |
8 hours |
|
How do you create cryosupernate and cryoprecipitate from FFP? |
Thaw at 1-6C and centrifuge |
|
How do you prepare usage of FFP? |
Thaw at in a 37 celsius waterbath |
|
How many hours should platelets be allowed to rest before placing in agitator? |
1-2 hours |
|
pH needed in platelet preservation |
6.2 |
|
the 2 types of platelet concentrate |
Random donor platelet concentrate and single donor platelet concentrate |
|
how many units of random donor platelet concentrate is needed to have a therapeutic effect? |
4-6 |
|
plt refractoriness |
no increase in platelet count |
|
quality control in single donor platelet concentrate |
3.0x10^11 platelets/unit |
|
QC in RDPC |
5.5x10^10 platelets/unit |
|
expiration date of pooled products |
4 hours |
|
After thawing FFP, within how many hours must you use it? |
24 hours |
|
After thawing cyroprecipitate, within how many hours must you use it? |
6 hours |
|
Leukocyte concentration expiry date |
24 hours |
|
1 unit of RBC can increase hemoglobin and hematocrit by? |
1 g/dL and by 3-5% |
|
contains all coagulation factors |
Fresh Frozen Plasma |
|
QC for Factor 8 in Cryoprecipitate |
80IU |
|
QC For Fibrinogen in Cryoprecipitate |
250mg |
|
PRBC should be transfused within how many hours/minutes after obtaining? |
4 hours |
|
Plasma products should be transfused within how many hours/minutes after obtaining? |
20 minutes |
|
Storage temperature and expiry: Whole Blood |
1-6C for 35 days |
|
Storage temperature and expiry: PRBC irradiated unit |
1-6C for 28 days from date of irradiation |
|
Storage temperature and expiry: RBC |
1-6C for at most 42 days |
|
Storage temperature and expiry: Leukocyte poor red blood cells |
1-6C Closed system: same for RBC open system: 24 hours |
|
Storage temperature and expiry: washed RBC |
1-6C for 24 hours (open system) |
|
Storage temperature and expiry: frozen RBC
|
-65C or -120C for 10 years |
|
Storage temperature and expiry: deglycerolized RBC |
for 24 hours |
|
Storage temperature and expiry: platelets random donor
|
20-24C with continuous agitation for 3-5 days |
|
Storage temperature and expiry: platlets single donor |
20-24C with continuos agitation for 3-5 days |
|
Storage temperature and expiry: FFP |
-18C for 1 year or -65C for 7 years |
|
Storage temperature and expiry: Single Donor Plasma |
1-6C for up to 5 days beyond whole blood expiration date |
|
Storage temperature and expiry: cryoprecipitate |
-18C for 1 year |
|
Storage temperature and expiry: Granulocyte Concentrate |
20-24C for 24 hours |
|
QC for granulocyte concentrate |
1x10^10 |
|
Blood component used: To restore oxygen carrying capacity |
whole blood or packed rbc |
|
Blood component used: to prevent TA-GVHD |
RBC irradiated unit |
|
Blood component used: washed RBC |
patients who have a history of plasma protein antibodies, diagnosis of PNH, history of frebrile reactions owing to luekoagglutinins |
|
Blood component used: leukocyte poor RBC |
history of reoccurring febrile reactions due to white cell antibodies |
|
Blood component used: platelets random donor |
to correct thrombocytopenia |
|
Blood component used: platelets single donor |
to correct thrombocytopenia from refractoriness |
|
Blood component used: FFP |
treatment of multiple coagulation factor deficiencies |
|
Blood component used: Single Donor Plasma |
treatment of stable clotting factor deficiency |
|
Blood component used: Cryoprecipitate |
Correction of Factor 8 and fibrinogen deficiency |
|
Blood component used: Granulocyte concentrate |
correct severe neutropenia, fever unresponsive to antibiotic therapy, and myeloid hypoplasia of the bone marrow |
|
High Glycerol technique freezing temperature |
-80 Celsius |
|
High glycerol RBC is stored at what temperature and where? |
-65 Celsius in a mechanical freezer |
|
Low glycerol freezing temperature |
-196 Celsius |
|
Low glycerol RBC stored at what temperature and where? |
-120 Celsius in liquid nitrogen |
|
Concentration for High Glycerol |
40% |
|
Concentration for Low Glycerol |
20% |
|
Slow freezing method for Frozen RBC |
High Glycerol |
|
Fast freezing method for Frozen RBC |
Low glycerol |
|
When is RhoGam given? |
28th week of gestation and 72 hours after birth |
|
Rhogam storage temp and expiry date |
1-6C for 3 years |
|
Deglycerolization |
Wash RBC in a hypertonic solution to isotonic solution |
|
1st generation filters for WBC |
Pore size is 170um and it removes fibrin clot |
|
2nd generation filters for WBC |
Pore size is 20-40um and it removes microaggregates |
|
3rd generation filters for WBC |
removes 99.9% of WBC |
|
US standard for QC of Leukocyte Poor RBC |
5x10^6 |
|
Choices for AB individuals from most preferred to least preferred |
AB, A, B, O |
|
Final check of ABO compatibility between donor and patient |
Crossmatching |
|
2 phases in crossmatching |
immediate spin and antiglobulin crossmatch |
|
Major crossmatching |
DR PS Donor's Red Blood cells Patient's Serum |
|
Minor Crossmatching |
DS PR Donor's serum Patient's red cells |
|
Sample for crossmatching |
Serum |
|
Sample for crossmatching should be obtained within? |
72 hours |
|
Donor and recipient serum must be stored at? |
1-6C for 7 days following transfusion |
|
Hemolyzed samples are allowed in Crossmatching. True or False? |
FALSE |
|
Unused units stored in the wrong temperature must be returned within how many hours or minutes? |
30 minutes |
|
Patients with fever cannot be given blood transfusion. True or false? |
true. fever is a sign of transfusion reaction |
|
Acute immunologic transfusion reactions |
hemolytic, febrile nonhemolytic, allergic, anaphylactic shock, and TRALI |
|
Acute nonimmunologic transfusion reactions |
bacterial contamination, circulatory overload, and physical/chemical hemolysis |
|
Delayed immunologic transfusion reactions |
hemolytic, TA-GVHD, posttransfusion purpura |
|
Delayed nonimmunologic transfusion reactions |
transfusion-induced hemosiderosis, disease transmission |
|
Anti-WBCs or anti-leukocytes are associated with what transfusion reaction? |
Febrile nonhemolytic reactionA |
|
ABO incompatibility is associated with what transfusion reaction? |
Acute hemolytic transfusion reaction |
|
High IgE levels are associated with what transfusion reaction? |
Allergic reaction |
|
Non-cardiogenic pulmonary edema and anti-WBC is associated with what transfusion reaction? |
TRALI |
|
Anaphylactic reactions are associated with what immunoglobulin? |
IgA |
|
Most common contaminants of platelet concentrate |
Bacillus, Streptococcus, Staphylococcus |
|
Iatrogenic transfusion reaction |
Circulatory overload or TACO |
|
Kidd blood group is associated with what transfusion reaction? |
Delayed hemolytic transfusion reaction |
|
Anti-platelet 1 is associated with what transfusion reaction? |
posttransfusion purpura |
|
Most suitable urine specimen for transfusion reaction investigation |
1st voided urine after transfusion |
|
What do you look for in urine in transfusion reactions? |
Hemoglobinuria |
|
Gel Technology advantage
|
requires no washing, and there is standardization of result |
|
Solid Phase technology advantage |
can use hemolyzed, lipemic or icteric sample |
|
Means to separate or to remove |
apheresis |
|
2 methods of apheresis |
intermittent, and continuous |
|
Apheresis involving two puncture sites
|
continuous |
|
Anticoagulant of choice for apheresis |
heparin |
|
Anticoagulant most commonly used in apheresis |
ACD |
|
Plasmapheresis donor should not have a protein below? |
6g/dL |
|
This is used to prevent hemosiderosis |
Plasmapheresis |