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16 Cards in this Set

  • Front
  • Back

What does Anti-Human Globulin (AHG) reagent contain?

Murine monoclonal Abs directed at relevant human proteins.

What is the purpose of the AHG test?

To test for the agglutination of Anti-D IgG Abs

What does Anti-D reagent contain?

The secretions of 2 human/murine heterohybridromas. There is no human serum component

What is the principle of the Anti-D reagent?

To detect the presence or absence of the D antigen. If negative test for weak D antigen

What does the gamma-clone control reagent contain?

Bovine Albumin

What is the principle of the Gamma-clone control reagent?

To test for flase positives in Anti-D

What is contained in the Anti-A, Anti-B, and Anti-A,B reagents?

Murine monoclonal and color, Anti-A patent blue, Anti-B naphthol yellow

What is the principle of the Anti-A, Anti-B, & Anti-A,B test?

To determine if the RBCs have A, B, or both A & B Ags

What is contained in the Reagent RBCs? (Reference cells)

Pool of Human O-type RBCs

What is the principle of the Reagent A1, A2, & B RBCs?

To check the patient's plasma for the presence of A1, A2, or B Abs

What does the Anti-D reagent contain that the Gamma-clone (Rh control) does not?

2 human/murine heterohybridromas

What are the controls for Anti-A, Anti-B, & Anti-A,B?

A1 and B cell tests for Ab

What does the Anti-A1 reagent contain?

D. biflorus, which mimics the Anti-A1 Ab reaction

What is the N-hance's intended use?

Use as a low-ionic-strength additive solution for antibody uptake

What is the principle of the N-hance reagent?

Creates a low-ionic-strength environment that increases the rate of Ab uptake during incubation, enabling incubation time to be shortened

What does the N-hance reagent use as a reagent?


A) sodium azide


B) Human O type cells


C) glycine

C) glycine