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15 Cards in this Set
- Front
- Back
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NON-viral methods of gene delivery include
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Cationic liposomes
Receptor-mediated polylysine-DNA complexes |
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Viral vectors in use include
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Adenoviruses
Retroviruses Lentiviruses Herpesviruses |
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Advantages of retrovirus vector
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Stable integration of genetic material
LOW immunogenicity |
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Disdvantages of retrovirus vector
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ONLY infect DIVIDING cells
Potential for "insertional mutagenesis" |
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Vector of choice for gene therapy of SCID
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Retrovirus
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Retroviral insertional mutagenesis that activates LMO2 causes
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Leukemia
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Advantages of adenovirus vector
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Capable of infecting dividing AND resting cells
Can be modified to infect only certain types of cells Conveniently grown to high amounts |
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Modification of what protein allows specific targeting of adenovirus vector?
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Fiber protein
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Disadvantages of adenovirus vector
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Genetic material does NOT integrate into host genome
Can trigger a significant immune response |
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Deletion of what gene renders adenovirus unable to replicate?
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E1A
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Removal of what gene allows insertion of more foreign DNA into adenovirus?
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E1B
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What genes are deleted in NON-replicating adenovirus therapy?
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E1A and E1B
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What does Fiber protein normally bind to?
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Coxsackievirus/Adenovirus Receptor (CAR)
NOTE: Remember, Fiber protein is modified to target adenovirus therapy |
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What is Suicide gene therapy?
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When adenovirus expressing herpesvirus TK is injected in a tumor
Pt. is then treated with Gancyclovir systemically Tumor cells expressing the TK gene are killed |
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"Oncolysis"
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Lysis of tumor due to replication/spread of virus only throughout tumor
E1B gene is deleted Cancer cells, (p53-) support viral spread Normal cells, (p53+) do not allow viral spread |
