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11 Cards in this Set

  • Front
  • Back

How far are enhancers bounded to in relation to the transcription region?



a) up to several kbs upstream


b) up to several kbs upstream/downstream


c) up to one kb upstream/downstream


d) up to one kb upstream


e) up to several kbs downstream

How far are enhancers bounded to in relation to the transcription region?



a) up to several kbs upstream


b) up to several kbs upstream/downstream


c) up to one kb upstream/downstream


d) up to one kb upstream


e) up to several kbs downstream

Where are promoters located relative to the start-site of transcription?



a) ~25 bp upstream


b) ~25 bp downstream


c) ~25 bp upstream/downstream


d) ~100 bp upstream/downstream


e) ~100 bp upstream

Where are promoters located relative to the start-site of transcription?



a) ~25 bp upstream


b) ~25 bp downstream


c) ~25 bp upstream/downstream


d) ~100 bp upstream/downstream


e) ~100 bp upstream

TATA boxes are required for RNAP docking



True


False



TATA boxes are required for RNAP docking



True


False - there are TATA-less promoters, TATA is mostly for eukaryotes only

RNAP I and RNAP II sequences are both generally well conserved between species



True


False


RNAP I and RNAP II sequences are both generally well conserved between organisms.



True


False - RNAP I is not well conserved

The core element and the UPE appear to be the most important promoter elements in the promoter region for RNAP I.



True


False

The core element and the UPE appear to be the most important promoter elements in the promoter region.



True


False



however, UPE absence still triggers basal transcription

What is the TATA sequence recognized by?



a) RNAP II


b) TAFs


c) TFs


d) TFIID


e) All of the above

What is the TATA sequence recognized by?



a) RNAP II


b) TAFs


c) TFs


d) TFIID - the TBP complex within TFIID


e) All of the above

TBP binds to TATA box by distorting DNA using:



a) Forming a hairpin loop


b) α-helix


c) Zinc fingers


d) β-sheet


e) a & d

TBP binds to TATA box by distorting DNA using:



a) Forming a hairpin loop


b) α-helix


c) Zinc fingers


d) β-sheet


e) a & d

TFIIH activities include:



a) Kinase


b) ATPase


c) Helicase


d) a & b


e) a & b & c

TFIIH activities include:



a) Kinase


b) ATPase


c) Helicase


d) a & b


e) a & b & c

Which of these is/are promoter element (s) (can be found either upstream/downstream)?



a) CAAT box


b) TATA box


c) GC Box


d) b & c


e) a & b & c

Which of these is/are promoter element (s) (can be found either upstream/downstream)?



a) CAAT box


b) TATA box


c) GC Box


d) b & c


e) a & b & c

Which one of these is not a step of RNAP binding?



a) TBP recognizes TATA box, TFIID is recruited along with TBP


b) TFIIA and TFIIB is recruited


c) RNAP II recruits TFIIF


d) TFIIH melts DNA by binding downstream of RNAPII and also phosphorylates tail


e) These are all correct


Which one of these is not a step of RNAP binding?



a) TBP recognizes TATA box, TFIID is recruited along with TBP


b) TFIIA and TFIIB is recruited


c) RNAP II recruits TFIIF


d) TFIIH melts DNA by binding downstream of RNAPII and also phosphorylates tail


e) These are all correct



ALSO IN CORRECT ORDER!

If a CAAT isn't a HAT then


Enhancers are promoters?



True


False

If a CAAT isn't a HAT then


Enhancers are promoter elementers?



True


False - no but enhancers are similar and contain similar elements