Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
10 Cards in this Set
- Front
- Back
|
The glaA gene in Aspergillus has a 18AA secretion sequence. Awamori variety used as high levels of extracellular secretion. Autolysis can take place the gain the cymosin from the bovine prochymosin B |
Ward N et al, Nature Biotechnology, 1990 |
|
|
Use of Kluveromyces lacis for expression of prochymosin. Uses LAC4 gene for beta galactosidase production as it's excreted from cell and is inducable by galactose or lactose. Leads to 100x greater expression. Better than saccharomyces as prochymosin can be directly secreted. |
Van Den Berg JA et al, Nature Biotchnology, 1990 |
|
|
Investigated the safety of genetically modified chymosin in K.lactis. Demonstrated alongside the papers that not only is the organism safe, but there is long evidence for it being used by man, and necessary care to handle it is already known and undertaken. |
Bonekamp FJ & Oosterom J, Applied Microbiology & Biotechnology, 1994 |
|
|
We have constructed a high-efficiency expression vector to direct the synthesis of heterologous polypeptides in yeast. The vector is termed a sandwich expression vector as the heterologous gene is inserted between the 5′ and 3′ control regions of the efficiently expressed yeast PGK gene. We have used this vector to direct the expression of three derivatives of the calf chymosin cDNA gene; preprochymosin, prochymosin and chymosin. Prochymosin is synthesised to at least 5% of total yeast-cell protein and furthermore, it can be readily activated to produce an enzyme which has milk-clotting activity. |
Emtage JS et al, PNAS, 1983 |
|
|
In this, adhE from Clostridium thermocellum was inserted. Coverts acetyl CoA through to ethanol. Although successful, levels were still low despite showing potential. 70% of the end product was ethanol |
Chung D et al, PNAS, 2014 |
|
|
Showed here the effectiveness of a fusion protein against a number of organisms expressed in both maize and rice. Gene responsible was a fusion protein of Bt toxin and and ricin b-chain protein. |
Mehlo et al, PNAS, 2005 |
|
|
Demonstrated that the addition of a number of genes into rice such as GNA from snowdrop lectins, Cry1Ac & others resulted in action against a number of insect pathogens such as "rice leaf folder", "yellow stemborer" |
Maqbool et al, Molecular Breeding, 2001 |
|
|
A-C-C-U-C-C could recognize a conserved sequence found in the ribosome binding sites of various coliphage mRNAs; it may thus be involved in the formation of the mRNA·30S subunit complex. |
Shine J & Dalgarno L, PNAS, 1974 |
|
|
A phytase plasmid containing the phytase gene (phyC) under the control of a phophaste depletion inducable promoter (pst-promoter) was inserted into B.subtilis BD170. On phosphate starvation, 5000x increase |
Vuolanto A et al, Biotechnology Letters, 2001 |
|
|
Addresses a number of issues when using algae as a system for biotechnology. States that current knowledge of many pathways is limited. Many are also not yet carbon neutral which they need to be to reap the real benefits of the technology |
Scott SA et al, Current Opinion in Biotechnology, 2010 |
