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4 Cards in this Set

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Gram staining

It is important that microbioligists can correctly identify bacteria that cause infections to enable them to decide the most effective treatment


Gram positive

If u see a purple stain when observing the smear under a microscope it shows the gram positive bacteria is present

Gram negative

If the smear has retained the pink safranin stain, this shows that gram negative bacteria are present. This is because their thinner cell walls and lipid membranes allow ethanol to wash off the crystal violet purple stain and to then retain the pink safranin stain

Practicle of gram staining

1 application of crystal violet


2 application of iodine


3 alcohol wash


4 application of safanin