Biology Grade 10 Unit 6/ Recombinant Dna Quiz

Front 1

What is recombinant DNA? (How many sources of genetic material does it consist of?)

Back 1

DNA material consisting of genetic material from 2 or more sources

Front 2

How many chromosomes do bacteria have?

Back 2

One

Front 3

What is a plasmid?

Back 3

A circular piece of DNA separate from the main chromosome in bacteria

Front 4

In recombinant DNA technology, plasmids can be used as _____

Back 4

Vectors

Front 5

Vectors move what from one cell to another?

Back 5

Genes

Front 6

What do you do to the plasmid in the first step of creating recombinant DNA?

Back 6

You isolate the plasmid from the bacteria

Front 7

Next, human ___ must be isolated from a cell

Back 7

DNA

Front 8

What is used to cut DNA containing the gene of interest from a chromosome?

Back 8

Restriction enzyme

Front 9

The cutting produces nitrogenous base overhangs and underhangs called _____

Back 9

sticky ends

Front 10

Nitrogenous bases _____ bond with each other

Back 10

Hydrogen

Front 11

To more permanently attach the gene of interest onto our plasmid, we use an enzyme called _____

Back 11

Lygase

Front 12

Bacteria are treated with _____ _____ to alter their cell wall and allow some to take up the plasmid

Back 12

Calcium chloride

Front 13

What are proteins that dissolve blood clots?

Back 13

Anticoagulants