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48 Cards in this Set

  • Front
  • Back

Transformation

process in which one strain of bacteria is changed by a gene or genes from another strain of bacteria

bacteriophage
virus that infects bacteria
nucleotide
monomer of nucleic acids made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base

base pairing

principle that bonds in DNA can form only between adenine and thymine and between guanine and cytosine

gene
sequence of DNA that codes for a protein and thus determines a trait
chromatin
granular material visible within the nucleus; consists of DNA tightly coiled around proteins
histone
protein molecule around which DNA is tightly coiled in chromatin
replication
copying process by which a cell duplicates its DNA
DNA polymerase
enzyme involved in DNA replication that joins individual nucleotides to produce a DNA molecule

Who is Fredrick Griffith?

He was a scientist who was trying to figure out why bacteria makes people sick, specifically phenomonia


What was Griffiths experiment?

He injected mice with disease causing bacteria.


He then heated the bacteria to kill it and then it didn't infect the mice.


Thus not being caused by chemical reaction, leading him to believe it was genes.


Who is Oswald Avery?

discovered that the nucleic acid DNA stores and transmits the genetic information from one generation of an organism to the next.


What is a virus?

a non-living particles, smaller than a cell, that can infect a living organism.

What is bacteriophage?

a virus that infects a bacteria

How do bacteriophage function?

they attach to the surface of a cell and injects genetic material into it and replicates quickly and finally bursts.

What is 32P (Phosphorus-32)

An isotope used to isolate DNA

What is 35S (Sulfur-35)?

An isotope used to isolate PROTEIN

What is Hershey-Chase experiment?

Used radioactive markers to label the DNA and proteins of bacteriophages.


They found Bacteriophages only inject DNA

What are the 3 parts of nucleotide?

Phosphorus group


Deoxyribose


Nitrogeneous Base

What are the four Nitrogeneous bases?

Adenine


Guanine


Cystosine


Thymine

What Nitrogenous bases are PURINES?

Adenine


Guanine

What Nitrogenous bases are Pyrimidines?

Cytosine


Thymine


What makes up the DNA backbone?

sugar phosphate

what type of bonds hold together nitrogenous bases?

hydrogen bonds

What did Erwin Chargaff discover about the relative quantities of nitrogenous bases?

they were always found in equal quantities


G + C


A + T

What did Rosalind Franklin used what technique to gain information about the structure of DNA?

used X-ray diffraction


purified DNA and then stretched the DNA fibers of glass and then x-rayed


What did Rosalind Franklins x-ray reveal?

Helical or helix shape

Watson & Crick learned what from Rosalind Franklin?

It helped them with their DNA model. showing them it was a helix that was wound with 2 strands around each other

Where is DNA found in the cell of Eukaryote?

cell nucleus


Where is DNA found in the prokaryotes?

in the cytoplasm


Eukaryotes have ________the amount of DNA as prokaryotes

1000

How many chromosomes do diploid human cells have?

46

Eukaryote chromosones contain both____ and _________

DNA and protein

DNA and protein are tightly packed together to form a substance called

chromatid

Chromatid consists of DNA that is tightly coiled around proteins called_______________?

histones

Together DNA and histone molecules form a beadlike structure called a _______________?

Nucleosome

Nucleosome pack with one another to form a thick fiber called a ____________?

coil

Coils in the Nucleosome then form a larger thick fiber called a _________________?

SUPERCOIL


During what stage in mitosis do the chromosomes become visible?

the tight packing phase

In prokaryote replication begins at ________ point and proceeds in __________ directions

single and 2

In Eukaryotes replication begins at _______points and proceeds in __________ direction

hundreds of places and proceeds in both directions

Replication is an important step in what phase of the cell cycle?

CELL DIVISION - duplication

What happens in DNA replication?

1. duplicates its DNA in a copying phase


2. DNA molecule divides into 2


3. Produces complimentary cells following base pairing.


4. Each strand of Double helix of the DNA serves as a template for the new strand

What does each strand of the double helix serve as for the new strand?

template

The place where replication begins is known as the ?

the unzipping of parent molecules when hydrogen bonds are broken

What is the primary enzyme involved in DNA replication. What does it do?

DNA polymerase. it joins individual nucleotides to produce a DNA molecule.

What is the enzyme called that unzips the DNA in preparation for replication?

Topoisomerase

What is the enzyme that glues the sugar-phosphate backbone together?

Ligase