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307 Cards in this Set

  • Front
  • Back

What is a monomer

Smaller units from which larger molecules are made

What is a polymer

A molecule made of many monomers joined together

Three examples of a monomer

Carbohydrate , amino acid, nucleotide

What is a condensation reaction

The process of joining two molecules by a chemical bond using the removal of water

What is a hydrolysis reaction

The process of breaking a chemical bond between molecules using water and energy

Name three monosaccharides

Glucose, fructose, galactose

What bond is formed between monosaccharides

Glycosidic Bond

Which monosaccharides is maltose made from

Glucose and glucose

Which monosaccharides is lactose produced from

Glucose and galactose

Which monosaccharides form sucrose

Glucose and fructose

What is a disaccharide molecule

A molecule made of two monosaccharides joined by a glycosidic bond

What is an isomer

Molecule with the same formula (atoms) but different arrangements

Difference between alpha and beta glucose

The OH and H

What is a polysaccharide

A chain of many monosaccharides joined by glycosidic bonds

How to test for a reducing sugar

Add benedicts reagent


Boil in water bath


Positive = changes from blue to red precipitate


Qualitative = colour


Quantitative = weigh precipitate, colourimeter

How to test for a non reducing sugar

Conduct test if first try is negative


Add hydrochloric acid


Heat in water bath


Neutralise with sodium hydrocarbonate


Repete benedicts regent test

What two molecules is starch made from

Amylopectin and amylose

Properties of starch that make is a good energy storage molecule in plants

Amylose = helix, compact for space and storage


Amylopectin = branched alpha glucose, easily hydrolysed to release energy quickly


Insoluble so does not affect osmosis

How to test of starch

Add iodine, if turns orange to blue then starch present

Why is glycogen a suitable energy storage molecule on humans

Highly branched alpha glucose for quick energy release


Highly compact for space

Why is cellulose good for plant walls

Long unbrached beta glucose form chains, weak hydrogen bonds form microfibrils. Strong for structure

Name two types of lipids

Tri glycride and phospholipid

What 4 molecules form a tri glycride

One glycerol and three fatty acids

What is the molecular formula of a fatty acid

What bond forms between a fatty acid and a glycerol molecule

An ester bond

Molecular drawing of a triglyceride

Difference between saturated and unsaturated hydrocarbon tails

Unsaturated = one or more double bonds between the carbon atoms


Saturated = no double bonds

Difference between phospholipid and triglyceride molecule

One fatty acid is replaced by a phosphate

Is the hydrocarbon tail of a lipid phydrophobic or hydrophilic

Hydrophobic

Main use of tryglycerides and why

Energy storage as higher energy than carbohydrates


Energy stores in bods, released when broken


Insoluble so don't affect osmosis


Hydrophobic tails so form droplets

Main use of phospholipid and why

Membrane due to hydrophobic tails and hydrophilic heads a bilayer is produced that acts as barrier + cells can controll what enters and exits

How to test for lipids

Add ethanol to solution


Shake


Add water


White precipitate = lipid present

What is a dipeptide

A molecule of two amino acids joined by a peptide bond

What bod is formed between amino acids

A peptide bond

What is a polypeptide

A chain of amino acids joined by a peptide bond

Molecular drawing of an amino acid

How many amino acids are there

20

What is the only difference between amino acids

Their variable R group

What are the four structural levels of a protein (names only)

Primary structure


Secondary structure


Tertiary structure


Quaternary structure

What is a proteins primary structure

The order and sequence of amino acids

What is a proteins secondary structure

Alpha helix or beta pleated sheet formed by hydrogen bonds

What is a proteins Tertiary structure

Protein is coiled and folded further due to formation of disulfide bridges, ionic bonds and hydrogen bonds, affects is shape and 3D formation

What is proteins Quaternary structure

Formation of several polypeptide held together by bonds affect the final 3D struture of a complex molecule

Four main functions of protein

Y (antibodies)


M (transport proteins)


C (enzymes)


A (structure)

How to use the biuret test for proteins

Make the solution alkaline add sodium hydroxide


Add copper (ll) sulfate solution


Purple of present blue if not

What is a catalysist?

A molecule that lowers activation energy required for reactions without being used up in the reaction itself

Why do enzymes lower the activation energy needed for reactions

Hold substrates together reducing repulsion so they can form a bond



Strains bond so they break more easily

What is the lock and key model

Enzymes active site eaxctally complementary in shape to the substrate

What is the induced fit model

Enzyme active sight is not complementary in shape to the substrate but changes shape when it binds to it

What is it called when and substrate fills an active site

Enzyme substrate complex

Why do enzymes only catalyse one reaction

Tertiary structure specific in shape to onw substrate, only forms E-s complexes with complementary substrate

What four things effect enzyme activity

Temperature


PH level


Enzyme concentration


Substrate concentration

Why does temperature affect enzyme activity

Increased kinetic energy more collisions + e-s complexes


Breaks hydrogen bonds permenantly denaturing active site

Why does pH affect enzyme activity

H+ ions temporarily break hydrogen bonds and denture enzymes

How does enzyme concentration effect rate of reaction

More enzyms means more collisions more e-s complexes formed, stops if substrates limited

How does substrate concentration effect rate of reactions

More substrate means more collisions and e-s formed untill saturation point when all enzyme active sites are filled

What does a competitive inhibitor do to the graph of rate of reaction

How does a competitive inhibitor affect reactions

Similar in shape to substrate, competes to fill active site, less e-s complexes formed reaction rate slows

Why at the end of the graph does the competitive inhibitor not make a difference

Increased substrate means more likely to collide to enzyme than inhibitor, percentage of solution Is too small to make a difference

What does a non competitive inhibitor do to the graph of rate of reaction

If less competitive inhibitor was added would the graph move up or down

Up

How does a non competitive inhibitor work

Binds to enzyme not at binding site


Alters Tertiary structure no longer complementary can't form e-s complexes rate if reaction plateaus

What two ways can you measure rate of enzyme controlled reactions

How much and fast product is produced


How fast the substrate is used

What does hydrogen peroxide break down into

Water and oxygen

How do you know what is the limiting factor of an enzyme controlled reaction

Whichever increases that causes the rate of reaction to increase is its limiting factor


Substrate. Enzymes.

Know how to create a tangent

Draw a triangle change in y over change in x

What does DNA stand for

Deoxy ribonucleic acid

What does RNA stand for

Ribonucleic acid

What is a nucleotide

A biological molecule, a monomer that forms polynucleotides

Draw a DNA nucleotide

What are the four possible bases of a DNA nucleotide

Tymine, guanine, cytosine, adenine

Draw a RNA nucleotide

Name four differences between a DNA and RNA nucleotide

Uracil not tymine


Ribose not deoxy ribose


DNA is long RNA is short


DNA is double strange RNA is single stranded

What are ribosomes made of

RNA and proteins

What does DNA do

Holds all genetic information

What does RNA do

Transfers genetic information from DNA to ribosomes

What type of reaction joins two nucleotides

Condensation

What is the bond between two nucleotides called

Phospodiester bond

Which parts form the sugar phosphate backbone

Pentose sugar and phosphate group

What bonds form between bases of DNA stands

Hydrogen

How many hydrogen bonds form between adenine and guanine

2

How many bonds form between cytosine and guanine

3

What is complementary base pairing

Bases can only join with one particular partner

Six important factors of DNA

Double stranded


Sugar phosphate backbone protects bases


Unidirectional only read one way


Antiparallell only join in opposite directions


Helix so compact


Codes for amino acids

Why did scientists think that proteins used to carry genetic information

DNA is simple and basic so was unlikely to be able to control such complexities

What type of replication does DNA replicate by

Semi Conservative

Why does DNA replicate

So that each cell has its own complete stand of DNA

Using keywords describe DNA replication

DNA helicase breaks bonds


Unwinds one becomes template stand


Free floating nucleotides attracted to exposed bases


Complematary base pairings


DNA polymerase condensation reaction to form phospodiester bonds


One template one new


Is DNA made from five prime to three prime or three prime to five prime

Five prime to three but template is read from three to five

What is an isotope

Different forms of the same genes

Draw a ATP molecule

Which enzyme forms ATP from ADP and Pi

ATP synthase

What molecules is ATP made from

ADP and pi

What type of reaction forms ATP from ADP and pi

Condensation reaction

What is released when ATP is hydrolysed

Energy ADP and pi

What enzyme causes ATP to form ADP and pi

ATP hydrolase

What does ATP and ADP stand for

Adenosine tri phosphate


Adenosine Di phosphate

Why is ATP hydrolysis couples with other reactions

Energy released can be used directly no. Lost as heat

What is phosphorylation

The addition of an inorganic phosphate added to a molecule to make it more reactive

Draw a simple structure of water

Name five reasons why water is important to organisms

It's a metabolite E.g condensation and hydrolysis reaction


It is a solvent so substances can disolve


Has a high heat capacity so can't heat up quickly


High latent heat of vaporisation so heat lost through sweat


Has strong cohesion allows for flow and tension


Name four inorganic ions and their charge

H+


PO 3- 4


Fe2+


Na+

What is an ion

An atom that has an electrical charge

What is an ion with a positive and negative charge called

Cation = pos


Anion = neg


What is an inorganic compound

Something that doesn't contain carbon

Why are iron ions important

Fe2+ in heamoglobin binds to form oxyheamoglobin

Why are hydrogen ions important

H+ determines pH level, lower = acidic

Why are sodium ions important

Transport glucose via Co transport


Nervous system

Why are phosphate ions important

Phoaporylate items to be more reactive


Forms ATP


Joins DNA strands

Name every organelle

Chloroplast


Golgi body


Smooth ER


Ribosomes


Lysosomes


Rough ER


Vacuole


Wall


Vesicles


Membrane


Nucleus


Mitochondria


Which one has a Nucleus prokaryote or eukaryote

Prokaryote

Things that a plant cell has but not an animal cell

Chloroplast chlorophyll stroma Vacuole wall plasmodrsmata

Cell wall of a fungal cell is made of what and what plant organelle don't they have

Chitin, chloroplast

How many chloroplast does a algal cell have

One

What is the cells membrane purpose

Controls what enters and leaves, displays receptor proteins

Name for mini things in a Nucleus

Nucleolus ( makes ribosomes)


Nuclear pour


Nuclear envelope


Chromatin

What is Mitochondria for in a cell and important structures in it

Site of aerobic respiration in the cell to release energy from glucose


Matrix = fluid


Crista = increase S.A

Draw a chloroplast

Use of the golgi body

Process and package lipids and proteins

What do Vesicles do

Store lipids and proteins and fuses with membrane to release them to blood

What's in Lysosomes

Lysozyme, enzymes

What is the function of a ribosome and what are they made of

Synthesises proteins, RNA and proteins

What does rough endoplasmic rectiluim do

Folds and processes proteins / ribosomes on it synthesise them

What does smooth endoplasmic rectiluim do

Synthesises and processes lipids

What is the use of a cell walk

Supports cell an prevents shape change

What is the use of a cell Vacuole what is its membrane calld

Maintains pressure isolates unwanted chemicals tonoplast

What is the term called when a cell is adapted for a specific function

Differentiation, specialisation

What is a tissue

Group of cells working together to preform a specific function

What is an organ

Grip of different tissues working together to preform a specific function

Five ways a prokaryote (bacteria) cell is different

Flafellum


Slime capsule


Cell wall


Circular DNA


No histone proteins


Plasmids

Draw a virus what does it have and not have

No plasma membrane, ribosomes or cytoplasm


Does have RNA capsid enzymes and attachment proteins

Explain what happens during binary fission

Circular DNA replicates


Moves to opposite poles


Cytokenisis


Two daughter cells different no of Plasmids

How to measure magnification

Magnification = image / real object

Two main types of eloctoscope

Optical light and electon

Why is optical microscope worse

Light is bigger wavelength


Needs to be thin


Smaller resolution


Smaller magnification


What cant you see in an optical microscope

Ribosomes, endoplasmic rectiluim and Lysosomes maybe Mitochondria

Why is electon microscope better

Better resolution


Higher magnification


Smaller organelles seen

What are the three stages of cell fractionation

Homogenisation


Filtration


Ultracentrifugation

Why are cells homogenised

To break open cell / membrane and release organelles

Why are cells filtered

To remove unbroken and large debris

Two parts of a centrifuged solution

Pellet = bottom


Supernatant = top

What order do the organelles get separated in during Ultracentrifugation

Nucleus, Mitochondria, Lysosomes, ER, robosomes

Why is a cold isotonic buffer used

Cold = reduced enzyme activity


Isotonic = prevents damage by osmosis


Buffer = prevents damage maintains pH

What are the four names of the the stages of mitosis

Prophase


Metaphase


Anaphase


Telophase

What happens during prophase

Chromosomes condense ( become visible)


Centroles move to poles release spindle


Nuclear envelope breaks down

What happens during Metaphase

Chromosomes with two chromatids attach / line up on spindle

What happens in Anaphase

Centromeres split cromatids move to opposite poles, create v shape

What happens during Telophase

Nuclear envelope forms , Cytokenisis

If of 10 cells of 100 going through mitosis are in Metaphase and the cell cycles is 15 hours how long is methaphase?

90 mins

Do cancer cells have slowed or uncontrolled cell devision?

Rapid uncontrolled

What do you put on a temporary mount

A cover slip

How to measure mitotic index / what is it

Percentage of cells in mitosis



Mitotic index = no of cells with vidible chromosomes / total no of observed cells

How to use a graticule

Graticule in eyepiece


Micro miter on stage


Calibrate Graticule ( find out how big one Graticule unit is in Um)


View specimen count how many Graticule units


Times by measures um

Why use a Graticule measurment

Can't have micrometer next to specimen


Different magnifications

Why are membranes fluid mosoac

Fluid as not fixed / constantly moving


Mosic as had proteins and cholesterol

Why is cholesterol in the membrane

To add structure and stability and restrict movement

What are the two types of electon microscope called

Transmission and scanning

Differences between transmission and scanning microscopes

TEM= electromagnets to focus through specimen, dencer means thicker


SEM= knocks off electrons, 3d surface image, thick specimens, Lower resolution

What is resolution

The ability to distinguish between two points

What is magnification

How many times bigger the object image can be made

During homogenization why is an isotonic cold buffer used

Cold reduced enzyme activity


Isotonic prevents water dameaf evy osmosis


Buffer maintains pH level

During cell fractionation what are the three steps

Homogenisation


Filtration


Ultracentrifugation

Why are cell homogenised

To break cells open / membrane and release organelles

Why are cells filterd before Ultracentrifugation

To remove large cell debris

Name 2 key words of Ultracentrifugation

Centrifuge


Pellet =bottom


Supernatant =top


Speed


Orger in which organelles arrive during centrifugation

Nuclei


Mitochondria


Lysosomes


ER


Ribosomes

Four stages of cell cycle

Mitosis, gap phase one, synthesis, gap phase two

What happens during gap phase one

Cell grows new organelles and proteins made

What happens during synthesis of the cell cycle

DNA replicated

What happens during gap phase two

Proteins needed for cell division made

What are proteins that go all the way though the membrane called and what's the other type

Intrinsic = through


Extrinsic = half

Draw the graph of membrane permeability vs temperature

Explain this graph

Below 0 deform and thaw = holes


Increase temp more kinetic energy


Above 40 denture and holes

What is simple diffusion

Passive net movement of particles from and area of higher concentration to an area of lower concentration through membrane

Difference between some and facilitated diffusion, and what types of molecules use facilitated

Use of proteins



Non lipid soluble


Big


Charged

Explain how a carrier protein works and what types of molecules use it

Molecule attaches to it


Changes shape


Releases onto opposite side


Large ones


Explain how a carrier protein works and its molecules

Diffuse down protein, charged molecules

Four things that affect the rate of diffusion

Concentration gradient


Diffusion distance / thickness


Surface area


Temperature

Two main things that increase facilitated diffusion rates

Concentration gradient


Number of proteins

Definition of osmosis

Movement of water from an area of higher water potential to an area of more negative water potential through a partially permeable membrane

Three factors affecting osmotic rate

Water potential difference


Thickness/ pathways


Surface area

Difference between an serial dilution and serial

Dilution series = all from original know pure concentration



Serila dilution = dilute each tube using solution from the previous tube

How to create a calibration curve and use it

Test know concentrations of something


Plot on graph name x,y axis


Plot points and draw line


Use graph to find corasponding units

What is active transport

The use of energy to move a molecule up its concentration gradient

Which protein is involved in active transport

Carrier

Differences between active transport and diffusion

Requires ATP / energy


Up concentration gradient

What are cotransporters and how do they work

Bind two molecules together movement of one molecule down its concentration gradient takes the other molecule up its concentration gradient

What factors affect active transport rate

Speed of proteins


No of proteins


Availability of ATP / respiration rate

How is glucose absorbed from the lumen

Sodium ions actively transported out of cell to blood


Creates concentration gradient


Joins to glucose brings it up its concentration gradient


Glucose diffuse into blood

Why are molecules digested before they can be absorbed

To large to go though membrane / proteins

What three enzymes break down carbohydrates

Amylase, maltase and membrane bound disaccaraidases

How does amylase digest starch

Hydrolysed glycosidic bond between monosaccharides using water

Where is amylase produced

Salivary glands, pancreas (realised to small intestine)

How do monosaccharides enter the ileum

Transporter proteins

How are lipids broken down

Lipase hydrolysed ester bonds to form fatty acids and monoglycerides

What is bikes role in lipid absorption and where is it produced

Emulsifiers lipids, nutalised stomach acid and produced in Pancras

How are lipids absorbed

Bike salts create smaller droplets have bigger surface area more area for lipase


Micilles formed diffuse though membrane

What three enzymes digest polypeptide

Endopeptidases, Endopeptidases and dipeptidases

How are amino acids absorbed

By cotransporters

What are antigens

Molecules / proteins that can trigger an immune response

The four stages of the immune response

Phagocytosis


Activate t cells


Activate b cells


Plasma cells

What happens in phagocytosis

Phagocyte recognises foreign antigen


Engulfs it to Phagocytic Vacuole


Lysosomes and lysozyme digest it


Becomes antigen presenting cell

What do phagocytes first stimulate

Helper t cells

What do helper t cells do

Receptor proteins on surface, bind to complementary antigen , activates the cell to stimulate phagocytes / cytoxic t cells / b cells

What do b cells do

Covered in antibodies, specific completer one binds, clonal selection, replicates to plasma cells clonal expansion

What do plasma cells do

Synthesise and secrete complematary antigens

Draw an antibody and what do they do

Bind to antigen, agglutination easier to engulf

I'v the immune responce what is cellular what is humoral

Cellular = phagocytes, t cells


Humoral = b cells monoclonal antibodies

What is the primary immune response and its traits

Body first exposed to antigen


Slow response as not many b cells


Symptoms felt


Memory cells created


What is a secondary immune response and its traits

Pathogen enters body


Memory cells recognise


Clonal selection + expansion = faster


Destroys pathogen before symptoms felt


More antibodies than primary exposure

How do vaccines work

Dead inactive pathogen / antigen


Immune response occurs


No symptoms felt


Immunity gained

What is antigenic variations and how does it help pathogens

Change surface antigens


Memory cells no longer recognize


Primary responses not secondary


Difficult to develop vaccines


Differences between active and passive Immunity

Requires exposure = active


No exposure = passive


Slow = active


Instant = passive


memory cells produced = active


No memory cells = passive


Long term protection = active


Short term = passive

How can monoclonal antibodies be used to treat specific cells

Proteins so unique Tertiary


Only completer to one surface antigen


Only bonds to cells with that antigen


E. G cancer

How do pregnancy tests work

Antibodies bound to beads


Pee added binds to beads


More antibodies fixed to plate


If pregnant then pee binds to both antigens blue beads remain and line seen

Describe the direct ELISA test and what does it stand for

Enzyme linked immunosorbent assay


Antibody attached to enzyme


Cells fixed to bottom of base


Base washed


If antigen present antibody and enzyme remain


Substrate added and if colour change then antigen present

Ethical issues surrounding vaccines

Tested on animals


Human testing is dangerous


Side effects


Epidemic who gets it is unfair

What does hiv and aids stand for

Human immunodeficiency virus


Aquired immunodeficiency syndrome

Draw a structure of hiv

How does hiv replicate

Attaches to surface protein on t cell membrane


Enters cell


RNA makes cDNA


Added to DNA


DNA produces genes and makes new hiv cells


Buda off with cell membrane and surface proteins

Why don't antibiotics work against viruses

Antigens on surface hidden by host cell membrane so not recognised


Don't have specific enzymes and ribosomes

What substances to organisms need to Exchange with the environment

Oxygen, nutrients, waste product, co2, urea same temp

Smaller animals have higher or lower suface area to volume ratio

Higher more of their surface on desplayed

Difference between bahavioural physiological adaptations to aid exchange

Size and shape = anatomical


Kidney effectivity = physiological


High energy foods = behavioural


Temperature of habitat = behavioural


Fur = anotatomical

Ways in which fish are adapted for gas exchange

Gill filaments and lamelae = surface area


Thing blood capillaries = diffusion distance


Counter current flow = maintains concentration gradient so o2 diffused into blood down whole length more effective

How insect are adapted for gas exchange

Pores called spiracles to teachea and tracheoles, thin + many = surface area and diffusion distance


Rhythmic abdominal pumping = moves air = maintains concentration gradient

Draw a cross section of a plant leaf

How insects minamise water loss

Close spiracles , waterproof waxy cuticle, thin hairs trap water

What are plants adapted to minamise water loss called

Xerophytic

Xerophytic adaptations

Sunken stoma


Hair


Curled leaves wind


Reduced stoma number


Waxy thick cuticle

Draw and labels lungs and mucles

Describe inspiration

Diaphragm contacts


External intercostal muscles contract


Increase volume


Decrease pressure


Air moves down pressure gradent


Active process

Describe expiration

External intercostal and diaphragm relax


Decrease volume


Increase pressure


Air moves down pressure gradient


Passive

Describe forced expiration

External and draphram mucles contact


Internal intercostal muscles contract


Smaller volume

How is the aveoile adapted for gas exchange

Thin one cell thick diffusion distance , many for surface area, ventilation maintains concentration gradient

Is endothelium in or out

Endothelium = the middle = in = blood capillaries

What is tidal volume

Volume of air each breath

What is Ventilation rate

Number of breaths per min

What is forced expiratory volume

Max volume of air breathed out in one sec

What is forced vital capacity

Max volume of air breathed out In one breath

How does a higher partial pressure of oxygen effect heamoglobin affinity for oxygen

Oxygen loads at an area of high partial pressure


Oxygen disassociate at an area of Lower partial pressure

Why is the o2 disassociation curve s shaped

Harder for first o2 to bind


Changes Tertiary structure


Easier for second and third to bind



Small change in pp affect affinity for O2

Which way does the disassociation curve move at a higher pp of co2

Higher pp of co2 means lower affinity so moves right

Draw the circulatory system and label Al the arteries and veins you need to

Needed = aorta


Phulmonary vein


Renal artery and vein


Vena cava


Pulmonary artery

Draw a cross section of an artery and label how it's adapted

Elastic tissue maintains pressure and controls volume


Draw a vein how us it adapted

Valves stop baxkflow helped by neighbouring muscle contraction

How is tissue fluid formed

Higher hydrostatic pressure, outward pressure forces water, o2, sugars out


Proteins and blood cells too big


Volume decrease pressure decreases


Osmotic effect water moves back on by osmosis


Excess fluid drained by lymphatic system

Draw a heart with labels

Describe the cardiac cycle not how a heartbeat is initiated

1 atria contract


1.1 av Vale open


2 blood moves to ventricles


2.1 av valve close


2.2 s.l valves open


3 artia relax ventricles contract from bottom


4 blood moves to arteries


4.1 s. L valves close


5 Both contact atria fill



Refer to pressure

What is an anthroma

White blood cells and lipids clump to form fatty streak. Endothelium increased resistance

What is an aneurysm

Balloon swelling of artery

What are the arteries around the heart called

Coronary arteries, myocardial Infarctuon is the blockage of them

Name 2 factors effecting cardiovascular desiese and how

High cholesterol more fatty acids and atheromas also salt equals higher pressure



Cigarette = high blood pressure and damage artery walls


What is transported in the xylem and phloem

Xylem is water


Pholem is solutes

Draw and lable the xylem

Describe transpiration

Cohesion of water molecules


Adehion to wall


Tension causes pull suction


Continuos water colum


Hydrogen bonds


Water evaporates from leaf


Four factors affecting transpiration rate

Wind = higher = continuous concentration difference so faster



Humidity = higher = smaller concentration difference = slower



Temp = higher = more energy so evaporate so faster



Light = higher = stoma open so faster transpiration rate

Draw a phloem and label it

Mass flow hypothesis

Solutes actively transported in by companion cells


Lowers water potential


Water moves in by osmosis


Increase pressure


Causes gradient moves down


Solutes actively transported out


Water moves out by osmosis

Evidence for mass flow

Ring of bark bulk


Radioactive labled carbon


Aphid heads cause drip


Metabolic inhibitor

Evidence against mass flow

Many sinks


Sive plates slow down progress

Differences between eukaryotic and prokaryotic DNA

Circular. Linear


No proteins, histone proteins


Plasmids, no Plasmids

What is a gene

Sequence of DNA bases that code for proteins

What is a genome

The complete set of genes in a cell

What is a proteome

The full range of proteins the cell is able to produce

Which one is used rxon a or intros

Exons bc Introns arnt in at the moment

What is an allele

For rent version of the same gene

What do you call two marching chromatids

Homologous pair

The gene is on the same what of two chromosomes

Locus

Draw transfer RNA

What happens during transcription

DNA helicase breaks bond unravels


RNA polymerase joins


Free floating RNA nucleotides


Complematary base pairing


Template strand


Exposed bases


Rna polymerase hydrolysed phospodiester bonds


Stop code reached


Disassociated


Pre mrna

What happens to pre mrna

It is spliced

What happens during translation

mRNA associates with ribosome


tRNA carries amino acids to it


ATP used to bind aa and tRNA


TRNA has anticodon complementary to base triplet it binds to


Aa joined by peptide bond


Formed by ribosome


Continues until stop code


Read many times

Which comes first translation or transcription

Transcription

Properties of the genetic code

Degenerate, many for one amino acid


Universal, sma win all organisms


Non overlapping, base only read once


Unidirectional, only reads one way

What are gametes

Haploid cells used for fertilisation

What happens in meiosis

Meiosis one, homologous pairs align in centre and move to opposite cells


Meiosis two, sister chromatids separated like mitosis


Two ways that meiosis causes variation

Independent segregation, homologous pairs align in any order



Crossing over, join at locus rejoin to doffent chromosome, New combination of alleles

How many chromosomes does a human have

46

As types of mutations

Substitution and deletion


Could be silent or not


Frame shift and degenerate

What are mutagenic agents

Increase rate of mutations


Smoking


Uv rays


Radiation

What is genetic diversity

Number of different alleles in a species or population

How is gone tic diversity increased

Mutations


Immigration

What is a genetic bottle neck

Event causes reduction In population


Reduced gene pool


Reduced genetic diversity


Reproduce frow a few individuals

Natural selection

Different reproduction success


Selection pressure


Advantageous alleles


Greater likelyhood of passing on alleles


Frequency increases


Advantageous allele becomes more popular

Difference between behavioural physiological and anatomical adaptations

Behavioural = play dead hide


Physiological = can't see kidney storage heart rate ect


Anatomical = see fur, blubber of fat

Two As selections

Directional selection


Stabalising selection

Directional selection

Individuals with extreme alleles and features more likely to survive E. G White in snow not grey or black


Others killed


More reproductive success


Selection pressure


More frequent

Stabalising selection

Middle range less extreme more likely to survive


Dora's advantage to be big or too small ect


Selection pressure


Advantageous alleles successful reproduction


Middle all frequency increase

Aseptic techniques

Prevent unwanted bacteria


Disinfectant in surfaces


Bunsen flame caries up


Sterilise in flame inoculation loop


Flame neck of bottle


Eyes gloves

What is phylogeny

Study of evolutionary history of organisms and how closely related they are

Basic shape of a phylogeny tree

Closer the branch the closer they're related

What is taxonomy

Science of classification involves naming them

The eight taxa names

Domain


Kingdom


Phylum


Class


Order


Family


Genus


Species

What is a hierarchy

Largest at top splits into smallest at bottom


Organisms only belong to one group no overlapping

What is a species

Group of similar offspring that can Reproduce to produce fertile of spring

How to do the binomial naming system

First part is the Genus has a capital letter


Second is the species always lowercase, italics or underlined

How can cortrship behaviours be helpful to species and classification

Attract same species


Mature fertile age


Colesly related more similar mating ritual

Three ways of clarifying evelotulionary relationship

Genome sequencing for similarities


Protein sequencing for similarities


Immunological responses

What is variation

Differences between individuals within a species or between a species

What is a standard deviation

How much the values in a sample vary

What is biodiversity

Variety of living organisms in an area

What is a habitat

Place where an organisms lives

What is a community

The populations of different species in a given habitat

Why is index of diversity better than species richness

Takes into consideration number of different species and poulation sizes


Higher the number the more diverse

How can agreculture reduce biodiversity

Woodland clearance


Hedgerow removal


Pesiticides herbicides


Monoculture


Remove niches and habitats

How to prevent loss of biodiversity

Legal protection


Protected areas