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19 Cards in this Set
- Front
- Back
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What are Porins?
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-large protein channels
-located in the Mitochondira -in the Outer Mitochondria Membrane (OMM) -when open: Mitochondria membrane is freely permeable |
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What is Cardiolipin?
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-it is a phospholipid
-found in the Inner Mitochondria Membrane (IMM) -Inner Mitochondria Membrane is rich in Cardiolipin |
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What does mtDNA do?
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-it is Mitochondrial DNA
-encodes polypetides that are integrated into: i. Inner Mitochondria Membrane ii. Ribosomes iii. tRNA - allows mitochondria to manufacture its own tRNA and proteins |
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What is in the matrix of the Mitochondria?
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-mt ribosomes
-mt DNA -high protein content with a gel consistency |
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What is the process of Apoptosis?
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1. Initiated by intracellular stimuli (i.e cell damage or failure)
2. Proapoptotic proteins trigger mitochondria to release Cytochrome C from (intermembrane space) 3. Cytochrome C triggers caspase which starts to break down cell and commits it to death. Once Cytochrome C is initiated the process cannot be reversed. |
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What 5 components make up the Cytoplasmic Endomembrane System?
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1. Endoplasmic Reticulum (ER)
2. Golgi Complex 3. Lysosomes 4. Vacuoles 5. Endosomal Vesicular Transport |
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Cisternae defintion
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Stacks of 'sacs'
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Proteins are synthesized, processed & secreted in the Biosynthetic / Secretory Endomembrane System.
True or False |
True
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What is GFP stand for?
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Green Fluorescent Protein - from the Jellyfish
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Explain the process of "Ectopic Expression of a GFP-tagged Protein"
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1.coding sequence of protein of interest is FUSED with coding sequence for GFP.
2. Fusion protein can be expressed in cells. 3. Fusion protein can be seen under a microscope and provides information about original protein Shows how things work and operate because you can see where it is. |
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Examples of Vesicular Transport:
i. Organelles to plasma membrane ii. Plasma membrane to organelles iii. Organelles to Organelles iv. ER to Golgi True or False |
True
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Characteristics of a Transport Vesicle
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-50-100 nm
-small, spherical, membrane-enclosed organelles -bud off donor compartment -fuse with acceptor compartment |
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Rabs
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TETHERING vesicle to target compartment
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Snares
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DOCKING vesicles to target compartment
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4 Steps of trafficking vesicles to a compartment
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1. Movement of vesicle using cytoskeleton & motor proteins
2. Rabs (protein) to tether vesicle to target compartment 3. SNAREs (protein) docks the vesicle to target compartment 4. Fusion of vesicle & target membrane |
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Organelle to Plasma Membrane is..
exocytosis or endocytosis |
Exocytosis
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Plasma Membrane to Organelle is..
exocytosis or endocytosis |
Endocytosis
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Give an example of Organelle to Plasma Membrane Exocytosis.
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-secretion of neurotransmitter
-secretion of collagen |
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Examples of secretions
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-saliva
-enzymes -mucos -hormones -neurotransmitters Anything a cell produces and releases is a secretion |
