Biochemistry Lab Exam 1

Front 1

What is the Henderson-Hasselbach equation?

Back 1

pH= pka+log([B]/[A])

Front 2

What are the steps to charge and mass balance?

Back 2

Set positive species equal to negative species
Set initial concentration of acid or base equal to the conc. of its conjugate
Use Kw=1e14=[H3O+][OH-]
Use quadratic formula to solve for unknown species

Front 3

What is the quadratic formula?

Back 3

(-b+or-(b^2-4ac)^(1/2))/2a

Front 4

What are the draw backs of the biuret method?

Back 4

Tris and ammonia interfere with the assay because they react with copper
The assay is insensitive

Front 5

What are the draw backs of the Lowry method?

Back 5

defeated by a variety of contaminants
low protein conc. needed
precise timing needed

Front 6

What are the draw backs of the BCA method?

Back 6

defeated by carbohydrates
color continues to develop over time

Front 7

What are the draw backs of the Bradford method?

Back 7

defeated by high conc. of detergents
stains cuvettes

Front 8

When is gel filtration used?

Back 8

to separate molecules according to Stoke's radius.
When the desired molecule is much larger or smaller than the others

Front 9

When is affinity chromatography used?

Back 9

To seperate proteins, nucleic acids, antibodies, and other biomolecules

Front 10

When is ion-exchange chromatography used?

Back 10

to purify ionizable molecules