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7 Cards in this Set
- Front
- Back
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DNA probe |
Southern Blotting (Edwin Southern) |
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RNA Probe |
Northern Blotting |
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Protein Identification by antibody |
Western Blotting |
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Solid phase DNA synthesis |
Synthetic DNA strand grown on small resin (solid phase). The new DNA strand is released (deprotected) from resin by rinsing it with Dichloroacetic Acid. |
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PCR (polymerase chain reaction) |
DNA is denatured, via increase in temperature. Brady resistant DNA polymerase are applied. Allow mixture to cool back down, then heat it back up (denature) again. Repeat process until desired amount of DNA is produced. No new products/ reactants added. |
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Recombinant DNA technology. |
Bacterial plasmids used as vessels to express various proteins. |
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Protein Expression |
Include promoter sequences to drive transcription. Organize the gene for proper translation. Poly blinkers aid in overall utility. |
