Biochemistry - Final Review - Ch. 3

Front 1

Column Chromatography

Back 1

Comprised of mobile and immobile phases. The compound of interest usually is attached to the immobile phase while the remaining molecular contents are filtered away.

Front 2

Molecular Exclusion Chromatography

Back 2

Separation by size: the immobile phase has small pores.

Large molecules emerge first, since the beads (immobile phase) only had pores small enough to catch the smaller molecules.

Front 3

Ion Exchange Chromatography

Back 3

Positively charged protein binds to negatively charged breads.

Negatively charged protein flows through.

The immobile phase is a charged resin. Bound molecules are rales by displacement generally with salt.

Front 4

Affinity Chromatography

Back 4

Glucose binding protein attaches to glucose residues on beads (immobile phase).

Glucose binding proteins are then released on addition of glucose.

This is a powerful purification technique based upon associations specifically with an individual protein.

Front 5

HPLC (High Pressure Liquid Chromatography)

Back 5

Smaller beads, faster and more complete separation.