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20 Cards in this Set
- Front
- Back
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"Coupled" Mitochondria
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-Fast oxygen consumption when ADP is present
-If remove ADP, ATP synthesis will stop and oxygen not consumed. |
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P/O ratio
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ATP/.5 oxygen consumed
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RCR tells us that we are only consuming O2 when making ATP
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(rate of O2 consumption w/ ADP)/(rate of O2 consumption w/o ADP)
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Product inhibition
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No ADP-->no ATP synthesis-->H will stay in the inner membrane space-->membrane potential gets too high-->proton pumping stops and ETC stops.
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Uncoupled mitochondria
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High oxygen consumption w/o ADP
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2,4 DNP
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-Example of an uncoupler
-Punches holes in inner membrane--> H leak back in-->no membrane potential--> no ATP. |
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When ADP removed and O2 utilization stops, what can be done?
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Add 2,4 DNP which causes 02 to be burned, but no proton gradient.
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Inhibition of mitochondria oxidative phosphorylation
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Oligomycin-blocks proton return through ATP synthase-->gradient continues to rise-->ETC stops
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Electron transfer inhibitors
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Everything stops and the uncoupler has no effect
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Complex I inhibitors
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Rotenone, amytal
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Complex III inhibitors
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Antimycin
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Complex IV inhibitors
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HCN, cyanide, H2S, CO, azide
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1. Are the mitochondria still consuming oxygen?
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Y--> 2,4 DNP
N--> Question 2 |
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2. Does 2,4 DNP addition restore oxygen consumption?
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Y--> Oligomycin
N--> Question 3 |
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3. Is there excess NADH and absence of QH2 (complex I)?
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Y--> Amytal, Rotenone
N--> Question 4 |
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4. Is there excess QH2 and absence of reduced Cyt.C (complex II)?
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Y--> Antimycin
N--> Question 5 |
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5. Is there excess of reduced Cyt c?
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Y-->HCN, Cyanide, H2S, CO, azide
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Specialized form of adipose tissue designed for controlled heat production?
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Brown fat mitochondria
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Brown fat mitochondria found in?
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Newborns, hibernating animals
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Thermogenin
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Inner membrane proton channel of mitochondria of brown fat.
Acts as a regulated "uncoupler" Leaks protons back into matrix w/o producing ATP. |
