Biochem Test 2

Front 1

∆E˚' is = to what?

Back 1

Eacc - Edonor

Front 2

Three ways to calculate alpha

Back 2

=Slope i / slope

=x-int / x-int i

=Km i / Km

Front 3

Three ways to calculate alpha prime

Back 3

= y-int i / y-int

= x-int i / x-int

=Vmax / Vmaxi

Front 4

Lineweaver-Burke Intercepts and slope

Back 4

m = Km/Vmax

y-int = 1/Vmax

x-int = -1/Km

Front 5

Changes in Km and Vmax by inhibition type

Back 5

Competitive affects Km, increases to show decreased S binding affinity. Only changes L-Burke slope and x-int

Uncompetitive affects both Km, decrease, and Vmax, decrease. Increases both y and x-int in L-B b/c they are both reciprocal.

Mixed, obviously both change. Look at direction of change.

Front 6

Limits on Steady state approximation

Back 6

When [ES] stays the same, b/c [S]>>[E]

Hope this provides a linear line of product formation [P]

Sketch w/ P,S,ES, and E.

Front 7

Noncompetitive inhibition

Back 7

When alpha = alpha'. Each affect Km in different directions so, Km is unchanged. Only Vmax changes.

Front 8

Assumptions to use Km as dissociation constant?

Back 8

k-1>>k2

Front 9

Assumptions for kcat?

Back 9

That one step is rate limiting.