Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
42 Cards in this Set
- Front
- Back
|
Enzyme Linked Immunosorbent Assay #1
|
Immobilize first antibody on solid support
|
|
|
Enzyme Linked Immunosorbent Assay #2
|
Incubate with protein sample
|
|
|
Enzyme Linked Immunosorbent Assay #3
|
Add 2nd antibody that is covalently linked
|
|
|
Enzyme Linked Immunosorbent Assay #4
|
Wash assay
|
|
|
Beer Lambert law
A= e= c= l= |
A=Absorbance/optical density
e=Absorptivity/extinction coefficient l=light path length (cm) c=concentration |
|
|
Dansyl Chloride
|
can help determine the N-terminus of a polypeptide
|
|
|
2-mercaptoethanol/mercaptans
|
cleaves disulfide links
|
|
|
Iodoacetate
|
prevents disulfide reformation
|
|
|
Trypsin cleaves:
|
Arg (R); Lys (K) (not before Pro P)
|
|
|
Chymotrypsin cleaves:
|
Tyr (Y); Threonine (W); Phe (F) (not before Pro P)
|
|
|
V8 protease cleaves:
|
Glu (E)
|
|
|
Cyanogen Bromide cleaves:
|
Met (M)
|
|
|
Lactose
|
Gal [beta 1,4] Glc
|
|
|
Sucrose
|
Glc [alpha 1,2] Fru
|
|
|
Cellulose
|
Glc [beta 1,4] Glc
|
|
|
Chitin
|
N-acetylglucosamine [beta 1,4] N....
|
|
|
Palmitic acid
|
HOOC(CH2)14CH3
|
|
|
Stearic acid
|
HOOC(CH2)16CH3
|
|
|
Oleic acid
|
HOOC(CH2)7CH=CH(CH2)CH3
|
|
|
Linoleic acid
|
HOOC(CH2)6(CH2CH=CH)2(CH2)4CH3
|
|
|
Ethanolamine
|
CH2CH2NH3+
|
|
|
Choline
|
CH2CH2NC(CH3)3+
|
|
|
Serine
|
CH2CH2(NH3)+COO-
|
|
|
Supercoiled DNA
|
L=T + W
|
|
|
Total result of glycolysis
|
2 pyruvate,
2ATP 2NADH |
|
|
Uniport ex:
|
valinomycin
|
|
|
Symport ex:
|
Lactose transport
|
|
|
Antiport ex:
|
Na+/K+ pumps
|
|
|
Desensitization effect
|
biological signaling systems adapt to long term stimuli by reducing their response to them <they respond to stimuli levels rather than absolute>
|
|
|
Carbonic anhydrous
|
CO2 + H20 > H+ + HC03-
|
|
|
Protein kinase
|
R2C2 + cAMP > 2C + R2 (cAMP)4
|
|
|
Myosin
|
ATP + H20 > ADP + Pi
|
|
|
Ca2+ ATPase
|
2Ca2+ >(ATP-ADP Mg2+) > 2Ca2+ + Pi
|
|
|
Pyruvate Kinase
|
....
|
|
|
Heterotrimeric G Protein
|
GTP + H20 > GDP + Pi
|
|
|
Ribosome synthesis (signaling pathway)
#1 |
Polypeptide synthesis begins
(mRNA, SRP, SRP receptor, Translocon, signal peptide) |
|
|
Ribosome synthesis (signaling pathway)
#2 |
Synthesis inhibited
|
|
|
Ribosome synthesis (signaling pathway)
#3 |
SRP docks to receptor to form ribosome translocon complex
|
|
|
Ribosome synthesis (signaling pathway)
#4 |
SRP and receptor dissociate... synthesis continues
|
|
|
Ribosome synthesis (signaling pathway)
#5 |
Signal peptide excised
|
|
|
Ribosome synthesis (signaling pathway)
#6 |
Protein grows, folds, and is modified by rough ER
|
|
|
Ribosome synthesis (signaling pathway)
#7 |
Ribosome dissociates
|
