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12 Cards in this Set

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  • Back

During transcription, how are RNA nucleotides added to the RNA transcript.

They are added in the 3' end of the transcript.





What direction is the DNA template strand being transcribed?

3' -> 5'

Can genes overlap?

yes

What are spliceosomes made of?

SnRNPs - SnRNA and proteins


and proteins

What is amino acyl tRNA synthetase?

enzymes that attach the correct amino acid to tRNA.




ATP -> AMP + 2pi

Where is the mRNA binding site?

At its 5' end. It binds to the 5' end of the mRNA.

What are the start and stop codons?

start = AUG


end = UAA,UAG,UGA

What is the function of the release factor in protein synthesis?

Release factor binds to the end codon in the A site and promotes polypeptide removing from tRNA (in the P site)

What is non sense mutation?

mutation resulting in early termination codon

What is a mis sense mutation?

One amino acid is changed (substitution)

What kind of cell is used in cloning?

mammary cells, they are totipotent (can give rise to all other cells) as they contain all the genetic information to make a clone.

How can cells protect themselves from mutation?

Nuclease cuts off the damaged strand.




DNA pol adds missing nucleotides and ligased together.