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160 Cards in this Set

  • Front
  • Back

Key Features of the Lab

Fire Extinguisher


Eye Wash


Shower


Non-hazardous Waste


Biohazardous Waste


Glass


Sharps Disposal

Compound Light Microscope

Blank Compound Light Microscope

Blank Compound Light Microscope

1. Ocular Lens


2. Head


3. Arm


4. Course Adjustment Knob


5. Fine Adjustment Knob


6. Light Control


7. Base


8. Substrate light


9. Mechanical Stage Control


10. Iris Diaphragm Lever


11. Condenser


12. Stage


13. Mechanical Stage


14. Objective Lenses


15. Revolving Nosepiece



The Stereoscopic Microscope

Making a Wet Mount

Blank Stereoscopic Microscope

Blank Stereoscopic Microscope

1. Eyepieces with Ocular Lenses


2. Magnification Control Knob


3. Head


4. Focus Adjustment Knob


5. Mirror


6. Stage


7. Objective Lens

Kingdom Monera


Bacteria

Tiny, single-celled prokaryotic organisms

•Very versatile


•Autotrophic or heterotrophic


•Reproduce asexually by binary fission – one cell duplicates and divides into two cells identical to the parent cell


•Individual bacterium microscopic, but colonies are visible to the naked eye

Bacteria Structure

Prokaryotic

lacks a nucleus &other membrane-bound organelles


•Possess a nucleoid that contains genetic info


•Genome consists of a single, large, circular DNA molecule


•Outer membrane, cell wall, and cytoplasmic inner plasma membrane


•Cell wall maintains shape of cell, protects, prevents bursting


•Cell wall is composed of peptidoglycan (large carb-protein polymer)

Bacterial Colony

An aggregation of daughter cells you can see with the naked eye

Cocci


Bacilli


Spirillia

Spheres- spherical shaped bacteria 
Rods- rod shaped bacteria 
Spirals- spiral shaped bacteria 

Spheres- spherical shaped bacteria


Rods- rod shaped bacteria


Spirals- spiral shaped bacteria

Cyanobacteria

Known as blue/green algae (but it's not algae!)


Aquaticphotosynthesizers


Oneof the largest groups in Kingdom Monera Canoccur as a single cell or as a colony


Genus:


Anabaena and Oscillatoria

Heterocyst


Specialized nitrogen-fixing cells      


Specialized nitrogen-fixing cells

Kingdom Protista


•Eukaryoticand mostly unicellular

What is the exception?


Brown, red and green algae!


Phaeophyta, Rhodophyta,and Chlorophyta•Verydiverse group of organisms


•Inhabitevery environment on Earth

Nutrition

Heterotrophs:


•protists ingest food by phagocytosis (ex.protozoa)


Autotrophic:


•protists sustain themselves using photosynthesis (ex.algae)


Mixotrophic:


•refers to a combination of methods usedto obtain nutrients

Classifying Protists

Movement:

•Flagella and cilia are appendages consisting of 9 outerdoublet microtubules and a pair of microtubules in the center


•What’sthe difference between the two?•Length!


•Pseudopodia: cytoplasmic extensions

Structure of Cilia and Flagella

Degree of Organization

Unicellular


Filamentous


Multi-nucleated


Multicellular


Colonial

Phylum Rhizopoda

Free living "Naked" Amoebas

Phylum Actinopoda

Phylum Rhizopoda

Phylum Foraminifera

Phylum Actinopoda

•contain internal shell composedof silicon dioxide; pseudopodia referred to as axopodiaand extend through pores in the test




•Radiolarians: marine organisms with fused shells

Phylum Foraminifera

•contain calcium carbonate testsand long spines to improve buoyancy


•Forams: found in warm tropical waters andreside on the ocean floor

Phylum Kinetoplastida

•flagellatedunicellular organisms containing a mitochondrion-kinetoplastcomplex containing extra DNA not housed in the nucleus



•Allows T. brucei tocause African sleeping sickness

Phylum Oomycota

•includeswater molds (freshwater decomposers) and white rusts/downy mildews (parasiteson terrestrial plants)

•Move using two flagella, no chloroplasts


Phylum Oomycota 

Phylum Oomycota



Phylum Kinetoplastida

Phylum Ciliophora

•Ciliacover entire body and/or cytostome

•Containone macronucleus (DNA & RNA synthesis, asexual reproduction) and 1+micronuclei (DNA synthesis, genetic variation)


•Trichocyst:long thread-like structure

Phylum Ciliophora

Phylum Apicomplexa

Parasitic,spread from one host to another as sporozoites and have no true means oflocomotion

•Causesmalaria, which is transferred by mosquitos


•Livesin the liver and blood cells of its host, can change surface proteins to trickimmune system•Merozoitesare produced asexually and burst RBCs, reducing hosts RBC levels

Phylum Apicomplexa

Slime Molds

•Similarto fungi, absorb nutrients from decomposing stuff

Phylum Acrasiomycota

•cellularslime molds that live as individual cells during vegetative stage

•Onlyaggregate when there is no food

Phylum Myxomycota

•calleda plasmodium when it is a multi-nucleated mass

•Vegetativestage: feeding


•Reproductivestage: no more food

Phylum Acrasiomycota
Phylum Myxomycota

Phylum Euglenophyta

•containsstorage molecule called paramylonand a flagellum

•Mixotrophic,free-living organisms


•Has an eyespot to help locate light

Phylum Dinoflagelleta


Phylum Pyrrophyta

•largerange of lifestyles, contain internal cellulose plates for protection

•Responsible for red tides

Phylum Chrysophyta



Diatoms

Silica shells consisting of two halves with pores for gas exchange

•Two body forms: pennate and centric


Golden-BrownAlgae


- Chlorophyll A&C plus other pigments


- Cell wall (if present) cellulose orpectin

Phylum Pyrrophyta (dinoflagellata)

Phylum Euglenophyta





Phylum Chrysophyta  
Diatoms 
Golden-Brown Algae  





Phylum Chrysophyta


Diatoms


Golden-Brown Algae

Phylum Phaeophyta


Brown Algae


•Fucoxanthinpigment gives color


•Thallus:seaweed body


•Holdfast:root-like anchor


•Stipe:stem-like structure


•Blades:leaf-like parts


•Algin isa multi-purpose polysaccharide extract •Examples:Laminaria,Fucus, Sargassum

Phylum Rhodophyta

Red Algae

•Phycocyaninandphycoerythrinaccessorypigments give reddish color

•Twocompounds in cell wall:


•Agar: culturing bacteria


•Carrageenan: commonly found intoothpaste, ice cream


•Example:Agardhiella

Phylum Chlorophyta

Green Algae


•Cholorphyllb asaccessory pigment


•Landplants and green algae are believed to have evolved from a common ancestor•Differentlevels of complexity


•Examples:Volvox, Hydrodictyon, Ulva

Phylum Phaeophyta


(Brown Algae)

Phylum Rhodophyta

(Red Algae)

Phylum Chlorophyta   (Green Algae) 
Phylum Chlorophyta

(Green Algae)



Fungi

Eukaryotic

Relatedmore to animals than plants


Prefermoist, dark environment


Heterotrophic


Absorption using exoenzymesto breakdown food outside of the body


Cellwall composed of chitin


Complex carbohydrate

Fungi Structure

Mostare multicellular

Exception to the rule?Yeast!


Composedof hyphae,or threadlike filamentsException to the rule?


Yeast AGAIN!


Hyphaecombine into a network (mycelium)that forms body of the fungus



Saprobes

Decompose dead organic matter intosimple nutrients

Symbiotic


Mycorrhizae


Lichens

Either parasitic or mutualisticpartners



Fungiand plant roots


Fungushyphae on plant’s roots increases surface area and helps absorb minerals andwater


Plantshares photosynthetic nutrients




Fungiin a symbiotic relationship with algae or cyanobacteria


Fungusprovides environment for the algae/cyanobacteria


Algae/cyanobacteriaprovide photosynthetic nutrients

Reproductions in Fungi

Sporesproduced either asexually (mitosis) or sexually (meiosis)

Adultfungi generally haploid


Diploidstage results when gametes of two different sexes combine to form a diploidzygotehares photosynthetic nutrients

Fungi Life Cycle



Foliose

Fruticose

Crustose

Mycota

Belonging to the kingdom fungi

Phylum Zygomycota

Zygote Fungi


Saprobes(Zygo) Sporangia:produce haploid (zygo) sporesvia meiosis

Phylum Ascomycota

Sac Fungi


Saprobicand parasiticMosthave a cup or sac-like appearance


Asci: produce haploid (asco)sporesvia meiosisConidiophores: produce haploidconidia/spores via mitosis

Phylum Basidiomycota

Club Fungi


Largephylum of well-known species with a club shape


Portabella mushrooms, toadstools, fairyrings, puffballs, shelf


Causediseases like wheat rust and corn smutStipe:stalk or stem


Pileus: capon top


Gillsunderneath contain basidia that produce (basidio)spores via meiosis

Phylum Deuteromycota

Imperfect Fungi


Not agroup based on shape or structure“Imperfect”= Only reproduce asexually


Causesmany diseases in humansAthlete’s foot, ringworm, yeast infectionCausesdisease in fruit – citrus mold

Phylum Zygomycota


Rhizopus


"Black Bread Mold"


Has fuzzy appearance



Phylum Zygomycota


Genus:


Pilobolus Coprophilous or dung-loving


"Shot-gun fungus"


Phototropic

Penicillium: Paintbrush

Importantin production of food and pharmaceuticals

Onespecies produces penicillinOthersused to make cheese

Penicillium 

Penicillium



  Phylum Ascomycota   
Phylum Ascomycota
  Phylum Basidiomycota   
Phylum Basidiomycota
Phylum Deuteromycota 



Phylum Deuteromycota

Lower Plants

•Growlower to the ground

•Allowsfor maximum contact with moist soils•Relyon osmosis, diffusion, capillary action etc. to absorb and move water from theenvironment to their cells


•Older,more basal or primitive plants


•Donot contain seeds or flowers found in common plant species

Alternation of Generations (1)

•Basiclife cycle of plants, two different life stages•Gametophyte(gamete-producing)

•Sporophyte(spore-producing)


•Dominance:one of these generations is more obvious, independent and photosynthetic•Lowerplants: gametophyte•Derivedplants: sporophyte

Alternation of Generations (2)

•Gametophytegeneration:

•Gametesproduced in structures called gametangia,protective function against desiccationofgametes


•Antheridia:male, sperm-producing •Archegonia:female, egg-producing


•Wheneggs are fertilized by sperm, become diploid zygotes and grow into youngdiploid sporophytes


•Sporophytegeneration:


•Haploidspores produced in structures called sporangia


•Germinatedspores form new haploid gametophyte plants

Life Cycle of Alternation of Generations

Gametophyte (n)

Producesgametes


nparents to ngametes


Mitosis


Producedin gametangia


Sporophyte (2n)


Produces spores


2n parents to n spores


Meiosis


Produced in sporangia


As a comparison…. in animals gametes are 2n parents to n gametes by meiosis

Transition from algae to land plants

•Twolineages of land plants diverged from algal ancestors

•Bryophytes– maintained gametophyte dominance


•Higherland plants – developed sporophyte dominance*9

Stonewarts


Genus: Chara

•Intermediatestep between green algae and land plants

•Havehigh calcium levels


•Differsfrom other green algae:


•Sexorgans located at tip of the plant and are multicellular


•Flagellatedsperm cells that fertilize eggs within gametangia

Stonewarts

Specialized Structures


Adaptive for Terrestrial Life

•Awaxy cuticle

•Pores/stomata


•Primitivewater and sugar conducting tissues

Division Bryophytes


Amphibians of the plant world

•Smallplants that grow in mats

•Onlya descriptive term, not a taxonomic category


•Threedifferent Divisions (phyla) represented, including Division Bryophyta(mosses) – don’t get confused!


•Lack vascular tissue


•Evolvedfrom algae in parallel with vascular plants


•Gametophyte generation is dominant•Dependenton water for reproduction, but cannot regulate water balance – can go dormantin drought conditions


•Sensitiveto pollution (indicator species)

Division Bryophyta


Mosses

•Haverhizoids thatanchor them into the substrate

•Nottrue roots, lack vascular tissue


•Sporophytegeneration consists of a capsule-shaped sporangium on a slender stalk (seta)•Short-lived,non-photosynthetic, depends on gametophyte for nutrition


•Somespecies of moss (Mnium) aremonoecious –both the antheridia and archegonia present on same plant


•Others(Polytrichum) aredioecious –separate male and female gametophytes



Division Bryophyta


Moss

Division Bryophyta


Mosses


•Phyllids:leaf-like structures that are spirally arranged on stem-like structures called caulids

Antheridia

Archegonia

Division Hepatophyta:


Liverworts

•Thallus isonly a few cells thick•Observepores and air chambers

•Canreproduce asexually


•Fragmentationof thallus


•Gametangia heldon slender stalks called gametangiophores

Division Hepatophyta:Liverworts

Division Anthocerophyta:


Hornworts

•Gametophytehas flat thallus


•Havelong, horn-like sporangium


•Meristem(area of active cell division) located in thallus


•Havetrue stomata inthe epidermis – different than the pores seen in liverwort

Division Anthocerophyta:

Hornworts

Seedless Vascular Plants

•Includesthe club mosses, horsetails, and ferns

•Have truevascular tissue –xylemand phloem•Cangrow larger, produce more gametes and spores


•Havetrue roots to absorb water


•Conservewater using stomata and a waxy cuticle


•Sporophytegeneration (2n) dominates •Gametophytegeneration more inconspicuous

Division Lycophyta


Club Mosses

•Canlive in moist habitats, Selaginella alsofound in deserts

•Haveclub or cone-shaped sporangia on stems: strobili (s. strobilus)


•Clustersof spore-bearing leaves called sporophylls

Division Lycophyta

Club Mosses

Division Sphenophyta


Horsetails

•Smallbrown scale-like leaves contain vascular tissue

•Cellwalls contain silica


•Containboth vegetative shoots (leaves) and spore-bearing shoots with strobili


•Novisible gametangia

Division Sphenophyta

Horsetails

Division Sphenophyta

Horsetails

Division Pterophyta


Whisk Ferns

•Psilotum

•Commonto Florida


•Lackstrue roots


•Stemsrise from a lateral, underground rhizome•Symbioticrelationship with a fungus

Division Pterophyta

Whisk Ferns

Division Pterophyta


Classic or "True" ferns

•Trueleaves called fronds (macrophylls)

•Young,curled up fronds called fiddleheads•Growalong underground rhizome (spreadsclones)


•Heart-shapedgametophyte(prothallus)also grows in substrate


•Darkfuzzy spots on fertile fronds: sori


•Clustersof sporangia

Division Pterophyta

Classic or "True" ferns

Division Pterophyta

Classic or "True" ferns

Life Cycle of Alternation of Generations Picture

Gymnosperms

•NakedSeeds

•Produce seeds, but no fruit


•Woody plants, long life cycles


•Characteristicsfor success on land:


–Small gametophytes:


•Multinucleated pollen (male); Femaleremains in ovule of sporophyte plant


•Gametes = resistant to desiccation


•Zygote protected = resides withinsporophyte plant


–Vascular tissue:


•Allows for transportation of water,nutrients, photosynthetic products

Angiosperms

•Flowering plants that produce fruit

•Phloem with sieve-tube members


–Efficienttransport of photosynthetic nutrients•Pollinated by wind, insects, birds, bats,etc.–Greatergenetic diversity


•Have reduced gametes


–Male- pollen grain


–Female- embryo sac = provides nutrition for embryo


•Endosperm= food storage

Gymnosperm Divisions

Coniferophyta(e.g., conifers, shrubs) Ginkgophyta (e.g., Ginkgobiloba) Onlysurviving species of this division)


Cycadophyta (e.g., Cycas,tropicalplants, 3 families)


Gnetophyta (e.g., Gnetum,Ephedra,3 families)•Allare wind pollinated


•Theyare heterosporous & monoecious


–Males produce microspores that arereleased for pollination


–Female produce megaspores to ovule housed in sporophyte plant



Gymnosperms Life Cycle

         
Ginkgophyta (e.g., Ginkgo biloba)
Cycadophyta (e.g., Cycas, tropical
plants, 3 families)
Cycadophyta

(e.g., Cycas, tropicalplants, 3 families)

Gnetophyta (e.g., Gnetum,Ephedra,3 families)

Coniferophyta(e.g., conifers, shrubs)

Strobili
cone-shaped sporangia
Microstrobilus


-Male
cone


-produces
microspores that develop into pollen

Microstrobilus


-Malecone


-producesmicrospores that develop into pollen

Megastrobilus


-Female
cone


-produces
megaspores which develop into the ovule (later become a seed)

Megastrobilus


-Femalecone


-producesmegaspores which develop into the ovule (later become a seed)

Angiosperms

•Unique Adaptations:


–Carpel= encloses the ovule àmatures into fruit


–Doublefertilization àsperm fuses with egg, other sperm fuses with 2 polar cells à endosperm


–Flowers


•Femaleand male reproductive structures which are surrounded by perianth(leaf-like)


•Protectgametes


•Ensurefertilization


–Attractpollinators (genetic diversity)

Angiosperms

Parts of a plant

Plant Diaphragm 

Plant Diaphragm

1. Pollen


2. Anther


3. Filament


4. Stamen


5. Sepal


6. Receptacle


7. Stem


8. Ovule


9. Ovary


10. Style


11. Stigma


12. Carpel


13. Petal

Plant Tissues

•Dermal tissue/epidermis: in contact withenvironment and offer protection, 1 cell thick


–Specializedcells: guard cells, stomata, root hairs


•Ground tissue


–Parenchyma:support/storage


–Sclerenchyma:support


–Collenchyma:support


•Vascular tissue


–Xylem:H2O & mineral transport


–Phloem:photosynthetic product transport


•Meristematic Tissue (mitosis for plant growth)


–Apicalmeristem: point on root/stem = growth


–Secondarymeristem: branches


–Vascularcambium: girth

Monocot

Roots: Fibrousroots that branch in different directions


Stem: Vascularbundles throughout


Leaves: Oneseed leaf; have floral parts in groups of three; Parallelveins

Dicot

Roots:


Central tap root that extends straight into the soil


Stem: Vascularbundles in a circle


Leaves: Twoseed leaves; have floral parts in groups of 4 or 5;


Nettedvenation

Monocot Stem cross section

Dicot stem cross section

Differences between Monocot and Dicot (angiosperms)


Roots, Stems, and Leaves

Monocot Root Cross Section

Leaf Structure



Dicot Root Cross Section

Stomata Structure

______ occurs when the mycelia of two different sexes come together and their cytoplasm fuses

Plasmogamy

Define Karyogamy

When the nuclei of two different organisms fuse

Name two things that Penicillium is used for:

Cheese


Antibiotic

What Fungi Phylum is not grouped based on their shape or structure but by the fact that they only reproduce sexually?

Phylum Deuteromycota

Lichen have a mutualistic relationship with other fungi.


True/False

True

Why are Bryophytes and Seedless Vascular plants referred to as "Lower Plants"?

Grows closer to the ground


Allowsfor maximum contact with moist soils


•Relyon osmosis, diffusion, capillary action etc. to absorb and move water from theenvironment to their cells


•Older,more basal or primitive plants


Donot contain seeds or flowers found in common plant species

The _____ tissue of a plant is composed of xylem and phloem

Vascular

Define Stomata

Pore that aids in gas exchange

In the sporophyte generation, haploid spores are produced in structures called ______

Sporangium

In one or two sentences describe the difference between a plasmodial slime mold and a cellular slime mold.

A Plasmodial slime mold is a mass of cell with many nuclei. (one cell, many nuclei)


A Cellular slime mold retains their cell walls and are an aggregate of cells. (multiple distinct cells).

Brown, red and green algae are examples of what three groups within Kingdom Protista?

Phaeophyta


Rhodophyta


Chlorophyta

What group within kingdom Protista is responsible for red tide events?

Dinoflagellates

The compound carrageenan comes from the cell walls of red algae and is often used in toothpaste and ice cream.


True/False

True

Malaria, caused by the protist____, finds its home in the ____and blood cells of its host.

Plasmodium


Liver

The five degrees of organization for protists are as follows:

1. Filamentous


2. Multinucleated


3. Multicellular


4. Unicellular


5. Colonial

Match the organism to the correct phylum:


A. Diatoms


B. Volvox


C. Naked Amoebas


D. Paramecium


E. Laminaria

1. Brown Algae; E


2. Ciliophora; D


3. Rhizopoda; C


4. Bacillariophyta; A


5. Green Algae; B

Trypanosoma Brucei is responsible for spreading what disease? What organism does it parasitize in order to spread?

African Sleeping Sickness


Tse Tse Fly

Members of Dinoflagellata use a flagellum to move, have eye spots in order to detect light and are mixotrophic.


True/False

False;


Phylum Euglenophyta

Define Trichocyst

The sharp blades beneath the surface of a paramecium's membrane that comes out when attached or when food is around.

Fungi are composed of threadlike filament known as______

Hyphae

All fungi are heterotrophic and eukaryotic.


True/False

True;


Except yeast (unicellular)

Describe the ideal environment for a fungus.

Dark and damp; but virtually everywhere

Define Mycelium

Body of the fungus

What is the name given to the mutualistic association of fungi and plant roots?

Mycorrhizae

What is the scientific name for a water bear?

Tardigrade

When placing a specimen on a compound light microscope, make sure to clean the lenses with a paper towel or Kleenex before examining.


True/False

False;


Use lens paper

The reason oil is used on the ____x objective lens is to prevent the scattering of ____

100


Light

What is the magnification of the ocular lenses in our microscopes?


What would be the total magnification be when looking through the 40x objective lens?

10x


400

A _____ microscope is used to view relatively large, thick specimens in three dimensions.

Stereoscopic

The three most common bacterial cell shapes are:

Cocci- spheres


Bacilli- rods


Spirillia- spirals

Put in order:


A. Add 5 drops of safranin, wait 20 seconds, rinse with tap water


B. Add 4-5 drops of iodine solution, wait one minute, rinse with tap water


C. Add 4-5 drops of crystal violet, wait one minute, rinse with tap water


D. Prepare smear


E. Add 3-5 drops of decolorizing alcohol solution, rinse immediately

1. D


2. C


3. B


4. E


5. A

Gram-positive bacteria have an outer lipopolysaccharide layer.


True/False

False;


Gram-Negative

Where is the genetic information found in a prokaryotes?

Nucleoid

______represents one of the largest groups in Kingdom Monera.

Cyanobacteria

Protists have been known to accomplish movement with the help of flagella, cilia and _____

Pseudopods

Define Mixotrophic

An organism that can make their own food (autotrophic) and also searches and eats food (heterotrophic). Has a mixture of both characteristics.