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24 Cards in this Set
- Front
- Back
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Benedict's Test detects... |
Reducing sugars |
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Benedict's Test Rxn |
Blue (no reducing sugars) --> red/orange (large amount of reducing sugars) |
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Benedict's Test Reagent |
Oxidized Cu |
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Needed for Benedict's Test to react |
A free aldehyde or ketone group |
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Benedict's Test Procedure |
- add Benedict's reagent to solution - HEAT solution in boiling water |
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Lugol's Test detects... |
Polysaccharides |
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Lugol's Test Rxn |
- Dark blue = positive - No change = negative |
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Lugol's Test Reagent |
Iodine |
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Needed for Lugol's Test to react |
Iodine binds to the alpha helix of polysaccharides |
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Lugol's Test Procedure |
Add Lugol's reagent |
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Sudan 4 Test detects... |
Lipids |
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Sudan 4 Test Rxn |
Top layer turns red |
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Sudan 4 Test Reagent |
Sudan 4 dye |
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Needed for Sudan 4 Test to react |
Sudan 4 dye is soluble in lipids, but not in water |
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Sudan 4 Test Procedure |
Add Sudan 4 dye |
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Biuret Test detects... |
Proteins |
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Biuret Test Rxn |
Turns dark blue = positive |
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Biuret Test reagent |
Biuret |
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Needed for Biuret Test to react |
Peptide bonds that connect amino acids to form proteins |
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Biuret Test procedure |
Add Biuret |
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Disaccharides have _____ bonds? |
Glycosidic bonds (covalent) |
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Polysaccharide has _____ bonds? |
Glycosidic bonds (covalent) |
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Triglycerides (lipids) have _____ bonds? |
Ester bonds |
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Amino acids (proteins) have _____ bonds? |
Peptide bonds |
