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18 Cards in this Set

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Restriction enzymes
cut DNA at specific sequences -- very important for manipulating
DNA in the lab.
Bacteria make restriction enzymes. The recognition sites for the cleavage are either 4 or 6
____ long.
bases
palindromic
DNA sequence of both strands when read in the same orientation (5' to 3') is the same
6 base recognition site
4096 bases 6^4
4 base recognition site
256 bases 4^4
_________ will not work on methylated DNA.
Restriction enzymes
When bacteria are infected by a ____, the _____ DNA enters the cell but is not
methylated. The restriction enzymes can chop up the _____ DNA and prevent it
from taking over the cell and turning it into a _____ factory.
phage
SNPs stands for:
single nucleotide polymorphisms
____ amplify
many regions with single nucleotide polymorphisms that affect restriction sites.
PCR
Agarose is derived from _____
agar
In an agrose gel, The _______charged DNA moves towards the ______ pole - _____ pieces move
_____ than longer ones.
negatively ; positive; shorter ; faster
RFLPs stand for
Restriction Fragment Length Polymorphisms
RFLPs can be used to identify:
people and track genetic linkage and inheritance
Plasmids
small circles of double-stranded DNA - 3,000 to 10,000 bases long
transformation
the insertion of a plasmid into a bacterial cell
overhangs
can hydrogen bond together and the strands can be sealed with DNA ligase enzyme
dideoxynucleotides are known as
chain terminators.
Reverse transcriptase
makes a DNA copy of RNA