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32 Cards in this Set
- Front
- Back
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the fact that bacteria are found everywhere, in almost every environment |
ubiquitous |
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TSA |
tryptic soy agar |
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TSB |
tryptic soy broth |
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Conditions of incubation that affect bacterial growth |
Time of growth
Availability of needed nutrients pH Salinity (salt concentration) Temperature |
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Why do we invert TSA plates and incubate them at 37 degrees Celsius? |
-so that moisture does not spill on the agar and mess the sample up -most bacteria grow best at body temperature |
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microbes are always present in aparticular area of the body
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resident flora |
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microbes that stay only for a short period of time and then move on or die off |
transient flora |
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The normal microbial flora has a ___________ relationship with the humanbody. That is, there is a close interaction between the two different organisms.
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symbiotic |
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helpful microbial flora |
mutalism |
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harmless microbial flora
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commensalism |
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potentially harmful microbial flora
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opportunism |
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Give an example of a bacteria that is mutalistic |
S. Epidermidis |
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Give an example of a bacteria with an opportunistic relationship with humans |
E. Coli |
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What was the predominant gram reaction and morphology of the normal flora?
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gram (+) cocci |
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The two most common methods of separating individual bacteria from one another are
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pour plates streak plates |
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purpose of preparing pour plates |
isolate individual colonies of bacteria quantify bacteria |
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pure cultures contain.... |
1 species of bacteria |
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What machine do we use in the lab to help accurately view the exact number of coloniesthat grew on a plate?
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Quebec Colony Counter |
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Tempering agar in 55°C water bath why?
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to cool the media but keep it a liquid so we do not kill the bacteria |
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How many colonies are on a countable plate? |
30-300 |
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TFTC |
too few to count (less than 30 colonies) |
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TNTC |
too numerous to count (more than 300 colonies) |
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NG |
no growth |
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Units for the quantity of bacteria in a sample |
CFU/ml |
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What type of plate is this and what is it used for? |
streak plate used to isolate colonies of bacteria |
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What type of plate is this and what is it used for? |
pour plate isolate colonies and to quantify bacteria |
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What is this type of plate and what is it used for? |
spread plate used for antibiotic sensitivity tests |
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Soap is formed via a simple process called |
soponification |
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Any cleansing solution (including soap) that removes bacteria embedded infats and oils.
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detergent |
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Organisms that can be removed by washing with soap or other detergent
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transient microorganisms |
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Organisms that reside in hair follicles and sebaceous glands of skin even after washing with soapor detergent
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resident microorganisms |
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Chemical agent that is applied to living tissue that prevents bacterialreplication, but DOES not kill the bacteria
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anticeptics |
