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57 Cards in this Set
- Front
- Back
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"On" State of GTPase |
Bound to GTP |
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"Off" State of GTPase |
Bound to GDP |
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GAP |
GTPase Activating Protein, Turns OFF |
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GEF |
Guanine Exchange Factor, Turns ON |
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The GEF for Ran |
RCC1 |
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Ran |
small G Protein involved in translocation through Nuclear Pore |
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Nuclear Pore cutoff (w/o Sequence) |
60 kDa |
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Ran-GTP is higher in the ____ |
Nucleus |
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Which Importin subunit binds to Ran-GTP? |
Beta |
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Ran GAP is in the ____, Ran GEF is in the ____ |
Cytoplasm, Nucleus |
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Experiment used to study Protein Shuttling |
Heterokaryon Assay |
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What determines Pore transport directionality? |
GTP gradient |
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Signal Sequence needed to send protein to Lysosome |
M6P |
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It is made by ____ in the _____ |
GlcNac Phosphotransferase, cis-Golgi |
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M6P binds to a receptor in the trans Golgi, it recruits ____ |
Clathrin |
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Lysosome hydrolases are ____ proteins |
soluble |
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Disease where GlcNac PT doesn't work, leads to lysosomal hydrolases being |
Inclusion Cell Disease; Secreted |
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Pathways using Clathrin |
PM to Late Endo; trans-Golgi to Late Endo |
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Pathways using COP II |
ER to cis-Golgi |
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Pathways using COP I |
t-Golgi to c-Golgi; c-Golgi to ER |
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G Protein that recruits COPI |
ARF1 |
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Arf1 is turned on by its GEF, _____ |
Sec7 |
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Sec7 is recruited by _____ |
KKXX |
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Type of ATP pump for Lysosome |
V Type H+ pump |
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Dissassembly of COP I proteins is caused by |
ARF1 GTP Hydrolysis |
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KKXX must be on the _____ side |
cytoplasmic |
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Transmembrane GEF protein on ER |
Sec12 |
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Sec12 activates _____ |
Sar1 |
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Sar1 anchors to membrane and forms complex with |
Sec 23/24 and later Sec 13/31 |
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Sec12, Sar1, Sec 23/24, Sec 13/31 are needed for the _____ vesicle |
COPII |
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COPI equivalent of Sar1 |
ARF1 |
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Clathrin proteins decoat upon ___ hydrolysis by ___ |
ATP, Hsc70 |
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To fuse, a vesicle must have a __ SNARE and a ___ |
V-, Rab |
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Rab Effectors are |
GAPs |
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Which proteins are needed to unbind the t/v SNARE complex? |
SNAP, NSF |
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NSF hydrolyzes |
ATP |
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A cytoplasmic loop has many ___ charges on the ____ side of the Transmembrane Domain |
+, N |
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Binds to the ER SS on a ribosome/nascent protein complex |
SRP |
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ON state for SRP and SRP receptor |
GTP bound |
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When SRP binds to its receptor, the ribosome and nascent protein bind to ____ |
Sec61 or Translocon |
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The ER SS is hydro_____ |
phobic |
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Glycosylation in the ER occurs on the _ terminal |
N |
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SRP is in the _____ |
cytosol |
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NLS and NES are _____translational modifications |
Post |
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A ligand receptor has a ___ binding site in the cytoplasm for endocytosis |
Adaptin |
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Adaptin binds to |
Clathrin |
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What pinches off the forming vesicle |
Dynamin |
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Dynamin is a __ase |
GTP |
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What decoats the vesicle |
Hsc70 |
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Hsc70 is a ____ase |
ATP |
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When the vesicle fuses with the endosome, what causes the receptor to unbind ligand? |
the pH sensor detects low pH |
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Ferrotransferrin binds to the transferrin receptor at pH ____, becomes apotransferrin at pH ___ |
7.0, 5.0-5.5 |
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____ and _____ are recycled |
Transferrin receptor and Transferrin |
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Na+/K+ is a ___ Pump that drives ___ AT |
ATP, primary |
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It is a _ Type pump |
P |
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Na/K ATPase fights the ____ effect |
Donnan |
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Two ways membranes can increase fluidity at low temperatures |
Unsaturating or shortening the hydrophobic tails |
