Bc 3 Rna Synthesis -- Splicing 1-2

Front 1

RNA synthesis is in _____________ while protein synthesis

Back 1

nucleus ____________________ the ribosomes (ER -- free)

Front 2

Three types of RNA

Back 2

tRNA, mRNA and rRNA

Front 3

RNA synthesis requires 3

Back 3

RNA polymerase, DNA template wt promoter region, from 5 to 3 direction

Front 4

Riframin ----------- Polymerase - TB - mito

Back 4

Bact RNA polymerase inhibitor ---- can interfere with mitochrondrial RNA ---> side effects --- only use to treat TB

Front 5

Alpha- Amanitin ---------- mushroom

Back 5

Mushroom toxin ---- affect RNA polymeratse 2 (fast growth cell) - -- GI disturbances - 40 -90% die within a few days

Front 6

either DNA can be _____________ strands depending on _________

Back 6

coding ---------- protein

Front 7

Overlapping genes happens a lot in ______________

Back 7

prokaryotes (bact - virus)

Front 8

There are 5 subunits _____________ of RNA polymerase and _______ is the only variable that directs to _________

Back 8

alpha, beta, gamma, Mr, sigma ------------------ sigma ------------ binding sites

Front 9

RNA polymerase binds at _________________ site which ____________ DNase to cleave and create _____________

Back 9

a specific ------------------ prevent -------------- RNA polymerase footprint`

Front 10

RNA polymerase consensus sequence ----- with specific sigma subunit binds to __________ xxxxxxxxxxxxxxxxx

Back 10

(UP element) ------------ -35 ----------------- - 10 ------ (AT rich -- ez to unzip) --- its corresponding promoter

Front 11

Rho independent termination ----------)start AT cut AU)

Back 11

AU rich --- make a loop at the end to terminate

Front 12

Polymerase code for _____

Back 12

pre- RNA

Front 13

TATA box

Back 13

similar to -10 of prokaryotic

Front 14

Polymerase 2 code for

Back 14

mRNA ---- recognize TATA box

Front 15

polymease 3 code for

Back 15

tRNA ---------- 5 S rRNA

Front 16

RNA polymerase 2 has a complex ------

Back 16

TBP (TATA binding protein) --------- TF (transcription factor) ------------- promote repair DNA and helicase activity - stop at Rho independent

Front 17

Actinoycin D -----is for D to R

Back 17

block DNA transcription to RNA

Front 18

Intron ---------- Exon

Back 18

non functional viral gene that can self splicing ------ coding sequense

Front 19

Primary transcript ------------------------- mature RNA

Back 19

with intron -------------- only Exon

Front 20

5'-cap ----------------------- Poly A tail

Back 20

protect RNA from degrading by RNase ------------ keep RNA last longer

Front 21

1st Group 1 intron --- cut to make

Back 21

no need ATP --- use Guanosine to cleave itself ---------- NUCleus - m-t-rRNA

Front 22

2nd Group 2 intron ----- only mRNA

Back 22

ATP ---- mito - chloro - mRNA - fungi - algae - plants

Front 23

Beta 0 -thalassemia -- start AT -- 0 --cant cut middle

Back 23

AT -- replace---- GT at 5' of 1st - 2nd intron -------------- splice junction at 3' end ----- snRNP can't bind -- can't cut

Front 24

3rd snRNP - an enz use to splice out RNA - eukaryote -- splieosome -- lariat

Back 24

ATP -- with A in the middle -------many U spliceosomes combine ---form Lariat and use A as nucleophile to cleave out intron

Front 25

Lupus erythematosus ----- snRNP --- chromatin - Phopholipid

Back 25

autoimmune attack snRNP --------- can't cleave intron out -------- chromatin - ribonucleoprotein --- cell membrane

Front 26

4th Enz intron removal

Back 26

2 ATP ------- endonuclease

Front 27

5 cap - includes

Back 27

3 P - 7 Met ==== 2 aldo Met to add 2 more Met

Front 28

Poly A tail

Back 28

AAUAAA --- add many AAA for protection

Front 29

Beta plus -thalassemia - cant cut tail

Back 29

mutatio AATAAA ----> AACAAA ---------> so can't cut off the poly tail ----------

Front 30

alternative splicing is

Back 30

throwing out different exon to have different gene product

Front 31

t RNA synthesis

Back 31

remove different portions ------ and modify the base by methylation (only Exon)

Front 32

polymerase 1 2 3

Back 32

1-all 2-Middle 3-Three