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22 Cards in this Set
- Front
- Back
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Clinical chemistry |
It is the branch of medical science involved in the analysis of biological materials , usually bodily fluids, to provide diagnostic results on the state of human body |
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Spectrophotometry |
The measurement of the intensity of light at selected wavelengths |
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Spectrophotometry |
A useful analytical tool to determine the concentration of colored material in a solution |
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Spectrophotometry |
Uses prisms or gratings to isolate a narrow range of wavelength of light |
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340-700 nm |
VISIBLE LIGHT |
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<340 nm |
Ultraviolet light |
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>700 nm |
Infrared light |
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Nephelometry |
Light scattered by an unknown substance is measured at right angles |
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Nephelometry |
Depends on wavelength and particle size |
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Turbidimetry |
Measures the amount of light blocked (absorbance) by a suspension of particles |
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Turbidimetry |
Depends on particle size and concentration |
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Electrophoresis |
Charged molecules moves at different rates when pulled through an electrical field |
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cathode (negative electrode) |
Cations (positively charged ions) will move to the _________ |
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anode (positive electrode) |
Anions (negatively charged ions) will move to the _________ |
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FLAME EMISSION SPECTROPHOTOMETRY |
Measures light emitted by excited atoms |
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FLAME EMISSION SPECTROPHOTOMETRY |
Certain elements give off a characteristic light after excited atoms return to ground state |
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Sodium |
intense yellow flame |
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Potassium |
violet flame |
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Calcium |
brick red flame |
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ATOMIC ABSORPTION SPECTROPHOTOMETRY |
Measures light absorbed by ground state atoms |
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ATOMIC ABSORPTION SPECTROPHOTOMETRY |
Routinely used to measure the concentrations of trace metals |
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ATOMIC ABSORPTION SPECTROPHOTOMETRY |
Hollow cathode lamp is the usual light source employed |
