Basi 1501-3 Lec 5 6

Front 1

Erythrocytes

Back 1

40-45% of bld volume

gas transport (o2, co2)

-surface area

Front 2

Erythrocytes at EM:

-lack _

-cytoplasm stains __

-flexi to deform thru tight spaces

-lifespan is _

Back 2

-typical organelles, NO NUCLEUS

-stains pink

-120 days

Front 3

Erythrocyte cytoskel defects:

-Spherocytosis:

-elliptocytosis:

-sickle cell

Back 3

-cant bend

-can bend but wont go back to orig shape

-cell is brittle, cant fit thru capillaries

Front 4

Leukocytes (WBC)

-are transported in periph bld BUT:

-fncn is:

-fncn happens in:

-life span: (cleaned up by macrophages)

Back 4

-dont fncn there

-host defence.

-happens in CT and interstitial spaces

-hours or days (short lived)

Front 5

Leukocyte types:

-

-

Back 5

-granulocytes (3 types)

-agranulocytes (2 types)

Front 6

3 granulocytes are :

-

-

-

Back 6

-neutrophil

-eosinophil

-basophil

ALL END IN PHIL

Front 7

2 agranulocytes are:

-

-

Back 7

-lymphocytes

-monocytes

END IN CYTES

Front 8

ALL Granulocytes have:

-_ granules (lysosomes)

-_ granules (enzymes w specific fncn) AKA specific granules

-have __ nuclei

-__rER and golgi (bc _)

-few _, depend on _ for E

-__lived

-are ALL between __ microns

Back 8

-primary

-secondary

-polymorphic/lobed nuclei

-poorly developed rER and golgi

-few mitochindria, depend on glycolysis for E

-short lived

- 12 - 15 microns

Front 9

Best way to ID Granuloctyes is by

-nucleus

-colour of specific granules

Back 9

-nucleus

-colour of spec granules

Front 10

Leukocytes - Granuloctyes - NEUTROPHILS

-__ of WBC (57-67% of WBC)

-fncn:

Back 10

-most numberous of WBC

-phagocytosis and destruction of BACTERIA

Front 11

Neutrophils at LM:

-nucleus:

-barr body

-cytoplasm stains:

-large primary _

-small, numerous _ granules (these are the __)

Back 11

-multilobed (2-5 libes)

-pale, neutral (can be light pink or purple)

-granules

-specific granules (these are the antibacterial agents!)

Front 12

Neutrophils at TEM:

-nucleus looks like __

-small, central __

-lysosomes are __

-specific granules vary in __ and are more __

Back 12

-cloud (multilobed)

-Golgi

-more e dense

-vary in density, more oblong than lysos

Front 13

Neutrophil how do they work:

-travel to __

-destroy _

-release _ (recruits more leukocytes, makes fever and inflamm)

-Is part of PUS

Back 13

-site of injury

-bacteria

-cytokines

Front 14

Leukocytes - Granulocytes - EOSINOPHILS

-very __ amount of WBC

-fncn:

-ppl with __ have lots of eosinophils

Back 14

-low

-destroy PARASITEs, phagocytose antigen-antibody complexes

-ppl with parasite infections or allergies

Front 15

Eosinophils at LM:

-nucleus:

-cytoplasm stains: _ so its _

Back 15

-BI-LOBED

-pink so its eosinophilic (acidophilic)

Front 16

Eosinophils at TEM:

-__nucleus

-__ specific granules

Back 16

-bi lobed

-CATS EYE (darker crystalloid body contains MBP which is antiparasitic)

Front 17

Leuko - Granulo - BASOPHILS

-least of WBC count

-fncn:

-can cause _

Back 17

-makes inflamm response and hypersensitivity rns

-anaphylaxis

Front 18

Basophil at LM:

-nucleus: __ but __

-cytoplams stains:

-primary granules are _

-specific granules: _

Back 18

-bi lobed but usualy obscured by specific granules

-stains DARK PURPLE (basophilic...)

-many of them (heparin, histamine, leukotrienes, chemotactic)

Front 19

Basophils at TEM:

-looks like _ nuclei

-__ specific granules

Back 19

loooks like two nuclei, bi lobed

-large big, e dense, specific granules

Front 20

How to tell MAST and BASOPHIL apart:

-in mast, nucleus is always

-in basophil, nucleus usually

-in mast, granules are _

-baso, granules are _

-Location:

---mast is in _

---baso is in __

Back 20

-round and in center (mast)

-obscured (baso)

-smaller (mast)

-larger granules (baso)

-mast is in CT

-baso is in periph bld

Front 21

Leukocytes - Agranulocytes:

-like all leukos, trvael in periph bld, diapedesis, host defense

-different from granulocytes bc:

-->no __ (but do have prim granules so lysosomes)

-->live __

-->nuclei are __

Back 21

-no specific granules

-long lived

-rounded/indented nuclei, no lobes

Front 22

Leukocytes - Agranulocytes - LYMPHOCYTES

-second most numerous behind __

-size: _(larger means more active)

-fncn: proper fncn of __ or __ (react with _, make _, attack _)

-nucleus:

-cytoplasm stains:

Back 22

-neutrophils

-7 to 16 microns

-lymphatic and immune system (react with specific antigens, make antibodies, attac foreign)

-huge nucleus, takes up most of cytoplasm

-stains light blue (just a rim)

Front 23

Lymphocytes:

-life span is :

-only WBC to __

-immunocompetent cells:

Back 23

-variable (days to years)

-to return to blood after being in CT or lymph

-able to recognize and respond to antigens

Front 24

Lymphocytes in TEM:

-huge __ (euchromatic)

-very few __

-few __

-dont have _

Back 24

-huge nucleus

-few mitochondria

-few lysosomes

-no specific granules

Front 25

3 types of lymphocytes (all look the same)

-B cells

-T cells

-Natural killer cells

Back 25

-B turn into plasma cells, make antibodies

-T do cell-med immunity, recruit macrophages, act against foreign cells

-NK innate immune response

Front 26

Leukocytes - Agranulocytes - MONOCYTES

-3 to 7% of WBC

-size:

-fncn: is __ to __

-nucleus: shaped like _

-cytoplams stains:

Back 26

-largest in size

-precursors to macrophages

-nucleus is like kidney bean or oval

-stains pale, greyish

Front 27

Monocytes in TEM:

-nucleus is __

-have _, small _ and _

-no specific granules

Back 27

-indented nucleus

-have golgi, small mitochon, and lysosomes

Front 28

Peripheral blood: Platelets (Thrombocytes)

-150-400k per mm3 (only 0.2% of bld volume)

-size:

-fncn:

-life span:

Back 28

-2-4 microns small

-makes bld clots and repairs vessel walls

-10 days

Front 29

Thrombocytes in LM:

-nucleus:

-cytoplasm stains __ in center, __ in periphery

Back 29

-no nucleus, just cell fragment

-dark in center, light on periphery

Front 30

Thrombocytes in TEM:

-shaped like

-has hyalomere and granulomere

-Hyalomere: marginal bundle has __ to maintain shape, open canicular system for __

-Granulomere: has granules specific for _, platelet aggregation , vaso_, stims repair of _. also has glycogen

Back 30

-microtubules and actin filaments, open canalicular is for more surface area

-coagulation, vasoconstriction, repair of vessel

Front 31

Where do bld cells originate? called hemopoesis

-initially:

-2nd trimester:

-3rd trim and life:

Back 31

-yolk sac

-liver and spleen

-bone marrow

Front 32

Bone marrow pictures:

-Section: can see __ and __

-Smear: __ those dots are __

Back 32

-can see bone and marrow

-big dark purple dots, small light purple dots

-thos dots are bld cells in dif stages of maturation

Front 33

Components of red bone marrow:

-Bone trabeculae

-__cytes

-hemo__ cords

-sinu__

Back 33

-adipocytes

-hemopoetic cords

-sunisoidal capillaries

Front 34

Red marrow structure:

-__ compartment: where _

-__ compartment: includes _

Back 34

-hemopoietic: where cells are made. arranged in islands and cords

-vascular: arteries, veins, sinusoids, all non-hemopoeitic cells