Bacterial Transcription Part 2 (4)

Front 1

Termination: The diagram below shows a GC-rich hairpin sequence of DNA followed by a stretch of A's. Why is this NOT an intrinsic terminator?

Back 1

The As are on the coding strand.

Front 2

Which of the following statements are TRUE regarding "€œPolarity" in terms of bacterial gene expression?

Back 2

It is when termination in a coding region located near the 5-end of a polycistronic mRNA causes the loss of both transcriptional and translational expression of all genes that follow it.

Front 3

In the diagram below showing bacterial RNA polymerase, what is indicated by the circle and arrow?

Back 3

circle of yellow strand which is coding strand

Front 4

What is required for a Rho-dependent terminator? (Note: inverted repeats in DNA form hairpins if transcribed into RNA.)

Back 4

an inverted repeat downstream of a very C-rich stretch of DNA

Front 5

In the diagram below showing bacterial RNA polymerase, what is indicated by the circle and arrow? (red area circled)

Back 5

template strand

Front 6

In the diagram below showing bacterial RNA polymerase, what is indicated by the circle and arrow? (goldish orange region)

Back 6

newly transcribed RNA

Front 7

What is the energy source that provides movement to Rho?

Back 7

ATP Hydrolysis

Front 8

What is the source of energy that allows an intrinsic terminator to function?

Back 8

thermal energy due to the temperature

Front 9

In the diagram below, which configuration would result in NO expression of coding region D at the translational level, but there would still be mRNA present?

Back 9

where the stop codon is at D aka 3

Front 10

In the diagram below, which configuration would result in NO expression of RNA encoding regions C and D?

Back 10

one

Front 11

Polarity: what condition must occur in order for a spontaneous STOP mutation to activate a Rho terminator embedded within a coding region?

Back 11

The STOP mutation must be located upstream of the rut site within the same coding region that contains the rho terminator.

Front 12

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Back 12

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Front 13

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Back 13

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