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23 Cards in this Set
- Front
- Back
what is automated analysis?
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mechanical or electrical control of a process that's repetitive,to minimize error and increase efficiency.
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what is continuous flow?
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-Continuous pumping of reagents into 1 rxn chamber;
-Specimens enter segmented flow at intervals. |
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How do automated analyzers meter out sample/reagent?
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with tubes of various diameters.
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what is contained in the stream?
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-Sample
-Reagent -Bubbles -Water wash between segments. |
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What can interfere with analyte msmt, and how is it removed?
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Protein - by dialysis.
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-How many components are in a continuous flow auto analyzer?
-What are they? |
Seven.
1) Sampler 2) Proportioning pump 3) Manifold 4) Dialyzer 5) Heating bath 6) Colorimeter 7) Recorder |
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What does a sampler do?
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aspirates samples, standards, and wash solutions in a timed sequence.
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what are the parts of a sampler?
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Cam, motor to turn the cam.
Sample/wash aspirator. |
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What is the heart of the system?
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Proportioning pump to move sample/reagent/air
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what does the manifold do?
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proportions, mixes, carries fluid through analysis by pump action.
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what is the dialyzer for?
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removing protein and interfering substances.
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what factors influence dialysis?
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-Time, Temp, Membrane type and Membrane surface. ALL CONSTANT.
-Concentration - the only variable. |
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How is the heating bath constructed,and why?
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So that solution flows from bottom up - gives time delay for ample color development.
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What happens to the stream before color measurment?
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DEBUBBLING.
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how does sample go through the colorimeter?
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through a flow cell.
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what 4 components are in the optical colorimeter for auto analysis?
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1. Tungsten lamp
2. Interference FILTER 3. 2 photocells - double beam in space. 4. Null balance circuit. |
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what type of recorder is used?
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a pen that draws a graph corresponding to color measurement. will reflect bubbles if present!
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what other photodetectors could be used?
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-Flame photometer
-Fluorometer |
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what is DISCRETE ANALYSIS?
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chemistry analysis of samples SEPERATED BY PHYSICAL BARRIERS.
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what is commonly used to seperate the samples?
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plastic envelopes with reagents in them.
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what is the Ektachem currently called?
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the Vitros
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what type of analysis does the Vitros use?
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-Dry slide technology with ion selective electrodes.
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what are the 4 key concepts re: the vitros?
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-Liquid free(dryslide) technol.
-Film slide -Reflectance spectrophotometry for opaque slides/samples -Air displacement sampling. |