Ascp

Front 1

Jembec plate

Back 1

MTM for Neisseria gonnorheae

Front 2

Enterobacteriaceae

Back 2

GNR
Oxidase negative
Ferment Glucose
Reduce nitrate to nitrite

Front 3

TSI - Salmonella typhi
TSI - Salmonella sp.
TSI - Shigella
TSI - Proteus vulgaris
TSI - Proteus mirabilis
TSI - Klebsiella
TSI - E. coli

Back 3

K/A no gas, H2S+/- no urea
K/A, gas, H2S pos no urea
K/A, no gas, no H2S no urea
K(or)A/A, gas H2S Pos UREA
K/A, gas, H2S pos UREA
A(or)K, gas, H2S neg UREA
A/K, +/- gas, no H2S no urea

Front 4

Haemophilus influenzae
Haemophilus parainfluenzae
Haemophilus hemolyticus

Back 4

Needs XV, -ALA
Needs V, +ALA
Needs XV, +ALA, hemolysis on quad

Front 5

Beta lactamase Test

Back 5

Nitrocefin disk (cephalosporin)
Positive: pink red
Negative: no color change

Front 6

X & V Requirements Test

Back 6

X factor: hemin
V factor: NAD

Front 7

DNase

Back 7

Positive= clearing Staphlylococcus aureus
Negative =

Front 8

Novobiocin susceptibility test

Back 8

S. saprophyticus = resistant = no zone of inhibition
CNS/ S. aureus = sensitive = 16 mm zone or larger

Front 9

Staph identification tests

Back 9

Catalase, DNase, Coagulase, novobiocin, Staphurex

Front 10

Streptococcus identification tests

Back 10

Catalase, Bile esculin, sodium chloride tolerance, Camp, Spot CAMP, Optochin, Bile solubility, Bacitracin, Hippurate hydrolysis, PYR, latex

Front 11

Camp

Back 11

Positive (arrow) = Group B Strep
negative = Group A strep

Front 12

Optochin susceptibility test (P disc)

Back 12

Selectively inhibits the growth of S. pneumoniae
Positive (zone) = Strep. pneumo
negative (no zone) = Strep viridans

Front 13

Bile solubility

Back 13

Disappearance of the tested colonies - hydrolyzed area
Positive = S. pneumoniae

Front 14

Bacitracin susceptibility test

Back 14

Positive (zone) = group A Beta hemolytic Strep
negative (no zone) = non-Group A Beta hemolytic Strep

Front 15

Hippurate Hydrolysis

Back 15

Enzyme hippuicase hydrolyses Na hippurate to benzoic acid and glycine
Positive (Purple) = Group B Strep, (gardnerella vaginalis, campy)
Negative (clear) = Group A Strep

Front 16

Plate CAMP Test

Back 16

Hemolytic activity of S. aureus is enhanced by extracellular protein produced by Beta Strep Group B
Arrow = Beta Strep GroupB
No arrow = Beta Strep group A

Front 17

Neisseria gonorrhoeae
Neisseria meningitidis
Moraxella

Back 17

Growth on MTM/Choc
Diplococci
Oxidase +

Front 18

Throat
Urine
sputum
wound

Back 18

Beta Strep A
S. aureus, S. sapro, E. faecalis, Enterobacteriaceae
S. pneumo, Pseudomonas, Haemophilus, Neisseria
Anything

Front 19

Gram Positive Bacilli

Back 19

Listeria monocytogenes
Erysipelothrix rhusiopathiae
Corynebacterium diphtheriae
Bacillus sp.
Lactobacillus sp.

Front 20

Listeria spp. tests (GPB)

Back 20

Bile - esculin

Catalase
Tumbling motility (@ RT)

Narrow zone of hemolysis

Front 21

Catalase + GPR

(4)

Back 21

Listeria monocytogenes
Erysipelothrix rhusiopathiae
Corynebacterium diphtheriae
Bacillus spp.

Front 22

Hydrogen Sulfide Production

Back 22

H2S reacts with iron salks to produce black precipitate ferric sulfide

Erisipelothrix rhusiopathiae + (only GPR +)

Front 23

Loeffler's Serum Medium

Back 23

Isolates C. diphtheriae but is non-selective
produces cells with metachromatic granules when stained with methylene blue stain

Front 24

Serum Tellurite agar

Back 24

Corynebacterium dephtheriae will appear as brown-black colonies with intense halo
*selective and differential

Front 25

Double zone of hemolysis

Back 25

Clostridium perfringens

Front 26

microscopic preparations for mycology

Back 26

Lactophenol cotton blue
Periodic acid-schiff stained smear
KOH prep

Front 27

C. albicans screen

Back 27

Positive: PRO+ and NGL+

Front 28

Germ Tube Test

Back 28

Positive: C. albicans, C. dublinensis
Negative: C. tropicalis

Front 29

Gram neg cocci

Back 29

Neisseria (diplo)
Moraxella (cocco)

Pasteurella

Brucella

Francicella

Front 30

D-ALA Porphryin Test

Back 30

ALA disc
Rxn: orange red fluorescence
Screens: H. influenzae, H. parainfluenzae, H. haemolyticu

Front 31

Bacitracin

Back 31

Bacitracin disc from blood agar
Rxn: zone
Screen: Beta Strep group A

Front 32

Esculin Hydrolysis Test

Back 32

Esculin Disc
Rxn: dark, no fluorescence
Screens: Enterococci +, Beta Strep group A neg

Front 33

Rapid hippurate Test

Back 33

Hippurate disc, nihydrin reagent
Rxn: purple color
Screens: Beta strep Group B, Gardnerella, Campy +

Front 34

Na Hippurate

Back 34

Rxn: Purple color in tube
Screens: Beta Strep Group B +, Beta Strep Group A neg

Front 35

Novobiocin Test

Back 35

Discs, 0.5 McFarland
Rxn: Zone
Screens: Staph. saprophyticus +, Coag Neg Staph - neg

Front 36

Optochin Test

Back 36

Taxo discs on Blood agar plate
Rxn: zone
Screens: Strep. pneumo, Strep viridans (group A) neg

Front 37

PYR

Back 37

Disc
Rxn: pink/red in 1 minute
screens: Enterococcus faecalis +, Alpha Strep viridans neg, S. lugdunensis +, CNST -

Front 38

Salt Tolerance

Back 38

Heart infusion broth
Rxn: growth
Screens: Enterococci faecalis +, Group D neg

Front 39

Plate CAMP test

Back 39

Blood agar, Staph aureus, beta Strep B, beta Strep A
Rxn: arrow head hemolysis
Screens: Beta strep Group B +, Beta strep Group A

Front 40

Group D

Back 40

Not entero: S. bovis

Entero: E. faecalis