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57 Cards in this Set

  • Front
  • Back

Microscopy

The process of using microscopes to magnify the image of an object.

Object

The material put under the microscope.

Image

The appearance of the material when viewed under the microscope.

Resolution

The minimum distance apart that two objects can be in order for them to appear as separate items.

Cell fractionation

The process where cells are broken up and the different organelles they contain are separated out.

Isotonic

Solutions that possess the same concentration of solutes and therefore have the same water potential.

Homogenation

When cells are broken up by a homogeniser (blender) which results in a fluid called homogenate.

Ultracentrifugation

The process by which the fragments in the filtered homogenate are separated in a machine called an ultracentrifuge.

Light microscope

A microscope which has poor resolution due to the relatively long wavelength of light.

Transmission Electron Microscope (TEM)

A microscope that focuses a beam of electrons onto the specimen. It must be in a vacuum, stained and extremely thin.

Scanning Electron Microscope (SEM)

A microscope that focuses a beam of electrons onto the specimen, which does not have to be thin (as it does in TEM).

Photomicrograph

A photograph of an image produced by a microscope.

Epithelial cell

They are eukaryotic cells, which have a distinct nucleus and possess membrane-bounded organelles.


Ultrastructure

The further, internal structure of a cell.

Nuclear envelope

A double membrane that surrounds the nucleus. The outer membrane is continuous with the ER of the cell and often has ribosomes of its surface. It controls the entry and exit of materials.

Nuclear pores

They allow the passage of large molecules, such as messenger RNA, out of the nucleus.

Nucleoplasm

The granular, jelly-like material that makes up the bulk of the nucleus.

Chromatin

The DNA found within the nucleoplasm. This is the diffuse form that chromosomes take up when the cell is not dividing.

Nucleolus

The small, spherical body within the nucleoplasm. It manufactures ribosomal RNA and assembles the ribosomes.

Mitochondrion

Rod-shaped in length and made up of a double membrane, cristae and matrix.

Double membrane

Surrounds the organelle. The outer one controlling the entry and exit of material. The inner one is folded to form extensions known as cristae.

Cristae

Shelf-like extensions of the inner membrane. These provide a large surface area for the attachment of enzymes involved in respiration.

Matrix

Makes up the remainder of the mitochondrion. It is a semi-rigid material containing proteins, lipids and traces of DNA for the production of its own proteins.

Rough Endoplasmic Reticulum (RER)

3D system of sheet-like membranes which has ribosomes present on the outer surfaces of the membranes.

Smooth Endoplasmic Reticulum (SER)

3D system of sheet-like membranes which lacks ribosomes on its surface and is often more tubular in appearance.

Golgi apparatus

A stack of membranes that make up flattened sacs, or cisternae, with small, rounded hollow structures called vesicles.

Lysosomes

When vesicles produced by the golgi apparatus contains enzymes, such as proteases and lipases.

Ribosomes

Small, cytoplasmic granules found in all cells. They may be found in the cytoplasm or be associated with the RER.

Microvilli

Tiny finger-like projections from cell-surface membrane of some animal cells to increase surface area for more efficient absorption.

Lipids

A varied group of substances that contain C, H and O. They are insoluble in water, but are soluble in organic solvents, such as alcohols and acetones.

Saturated

A fatty acid in which there are no double bonds between the carbon atoms.

Mono-unsaturated

Fatty acid that possesses a carbon chain with a single double bond.

Polyunsaturated

Fatty acid that possesses carbon chains with many double bonds.

Phospholipids

Triglycerides in which one of the three fatty acid molecules is replaced by a phosphate molecule. Phospholipids are important in the structure and functioning of the membranes.

Hydrophilic head

Interacts with water but not with fat.

Hydrophobic tail

Orients itself away from water, but mixes readily with fat.

Polar

Molecules that have two ends which behave differently (lipids).

Plasma membranes

Surrounds the cell and forms a boundary between the cell cytoplasm and the environment.

Extrinsic proteins

Occur either on the bilayer or partly embedded on it. They give mechanical support to the membrane or as cell receptors for molecules, such as hormones.

Intrinsic protiens

Completely span the phospholipid bilayer from one side to the other. Some act as carriers to transport water-soluble material across the membrane while others are enzymes.

Fluid-Mosaic model

Where individual phospholipid molecules can move relative to one another (fluid) and when proteins in the bilayer vary in shape, size and pattern (mosaic).

Diffusion

The movement of molecules or ions from a region where they are in high concentration to one where their concentration is lower.

Facilitated diffusion

Diffusion involving the presence of protein carrier molecules to allow the passive movement of substances across plasma mebranes.

Osmosis

The passage of water from a region of high water potential to a region where its water potential is lower, through a partially permeable membrane.

Water potential

The pressure created by water molecules. It is the measure of the extent to which a solution gives out water. The greater number of water molecules present, the higher (less negative) the water potential. Pure water has a water potential of zero.

Active transport

Movement of a substance from a region where it is in low concentration, to a region where its concentration is high. The process requires the expenditure of metabolic energy.

Villi

Finger-like projections of the intestinal wall, which increases the surface area for more efficient absorption.

Absorption

Uptake of substances.

Lumen

The hollow cavity inside a tubular structure, such as the gut or a xylem vessel.

Sodium-potassium pump

The mechanism by which glucose is absorbed from the small intestine.

Carrier protein

A protein on the surface of a cell that helps transport molecules and ions across plasma membranes.

Cholera

An infectious disease characterised by dehydration, watery faeces (diarrhoea), vomiting, etc.

Cell wall

Physical barrier that protects against mechanical damage and excludes certain substances.

Capsule

Protects bacterium from other cells and helps groups of bacteria to stick together for further protection.

Flagella

Aids movement of bacterium because of its rigid, corkscrew shape and rotating base that helps the cell spin through fluids.

Plasmids

A small, circular piece of DNA found in bacterial cells.

Diarrhoea

An intestinal disorder in which watery faeces are produced frequently.