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72 Cards in this Set

  • Front
  • Back
Are viruses living or non living?
Non living
Misfolded versions of normal proteins
Prions
Viruses are composed of these four things
1) Capsid
2) Nucleic acid
3) Envelope
4) Tail fibers
Protein coat (either rod shaped, helical, polyhedral)
Capsid
On animal viruses to help it infect the host
Envelope
For attachment and injection of nucleic acid
Tail fibers
Viruses _____ instead of reproducing
Replicate
Two major cycles of viruses :
1) Lytic Cycle
2) Lysogenic Cycle
Steps of the Lytic Cycle :
Attachment, injecting nucleic acid into host cell, break down host DNA and replicate viral parts, assembly of parts, lysis of cell
Steps of the Lysogenic Cycle :
Attachment, injects, recombination (Viral DNA joins host DNA), bacterial cell reproduces by binary fission, activation of lysogenic phage, kicks into lytic cycle
Two types of genetic recombination in bacteria
1) Transformation
2) Transduction
Gene transfer during which a bacterial cell uses foreign DNA from surroundings
Transformation
Gene transfer from one bacterium to another by a bacteriophage
Transduction
Random pieces of host DNA are packaged in the capsid during the lytic cycle (Type of transduction)
Generalized
Prophage snips out piece of host DNA and carries it with it. Only occurs in temperate phages (Type of transduction)
Specialized/restricted transduction
Conjugation forms a _____ between two bacteria and DNA is transferred
Bridge
Larger double stranded loops of DNA in conjugation
Plasmids
Smaller loops of DNA in conjugation
Episomes
F plasmids
Fertility
F+
Male with pili + plasmid
F-
Female
R plasmids
Drug resistance gene
Jumping genes are also known as
Transposons
Who discovered transposons
McClintock
Genes switch on and off as conditions in the intracellular _____ changes
Environment
What are the two main ways bacteria control genes switching on and off?
1) Regulation of enzyme activity
2) Regulation of gene expression
Regulation of enzyme activity means
Feedback inhibition
Regulation of gene expression means
Accumulation of product may trigger a mechanism that inhibits transcription of mRNA production by genes that code for an enzyme at the beginning of the pathway
Who proposed the operon concept
Jacob - Monod
What are the Operon components?
1) Operon
2) Structural genes
3) Operator
4) Regulatory genes
Structural genes with related functions and promoter region with operator
Operon
Code for a polypeptide, enzyme
Structural genes
Acts as on/off switch for transcription of the structural genes
Operator
The operator is ON unless the _____ is present to switch it OFF
Repressor
Code for repressors and regulators
Regulatory genes
Transcription of regulatory genes produces mRNA translated into regulatory protein that binds to the operator that represses or activates _____ of operon's _____ _____
1) Transcription
2) Structural genes
Transcription of regulatory genes produces mRNA translated into regulatory protein that binds to _____ which represses or activates transcription of operon's structural genes
Operator
Transcription of regulatory genes produces mRNA translated into ____ _____ that binds to operator which represses or activates transcription of operon's structural genes
Regulatory protein
Transcription of regulatory genes produces _____ translated into regulatory protein that binds to operator which represses or activates transcription of operon's structural genes
mRNA
_____ _____ binds to the promoter and moves past the operator to transcribe the structural genes unless the repressor protein is in the operator
RNA Polymerase
In the trp operon, the presence of tryptophan acts as a _____ when it binds to the repressor protein and both move into the operator turning the operon off
Corepressor
Lactose binds to repressor protein to inactivate it so the enzyme to break lactose down can be transcribed and translated. Said to be an _____ _____
Inducible enzyme
Are the promoter and operator transcribed?
NO!!!!!
Cause plant viruses
Viroids
Small molecules of naked RNA that disturb the metabolism of a plant cell
Viroid
Viroids may spread from plant to plant by these two ways
1) Horizontal transmission
2) Vertical transmission
External source like a gardener's tool, animals, cross contamination
Horizontal transmission
Inheritance of a viral infection from a parent plant. Asexually by propagation or sexually by infected seeds
Vertical transmission
What is the difference between horizontal and vertical transmission?
Horizontal is getting the viroid by an external source (objects), while vertical transmission is inheriting the viral infection from a parent plant
Carriers of viruses
Vectors
_____ _____ usually stunt plant growth
RNA viruses
Genes that trigger cancerous transformation in cells
Oncogenes
Substances that can trigger oncogenes/cause cancer to occur
Carcinogens
A weakened derivative of the pathogen that triggers immunity
Vaccine
Abnormal growth of cells resulting in a mass
Tumor
Not generally life-threatening; contained at the site (tumor)
Benign
Tumor that may be life-threatening; cells have spread from the original mass
Malignant
Cancer has spread to other areas of the body
Mestastasize
Viruses are possibly evolved from fragments of cellular nucleic acid that acquired special _____
Pkg
Viruses possibly originated from _____ that never re-entered the sequence
Transposons
Three most common cell shapes
1) Cocci
2) Bacilli
3) Spirilla
The cocci cell shape is _____
Spherical
The bacilli cell shape is _____ _____
Rod shaped
The spirilla cell shape is _____
Helical
Cell walls are made of _____, or polymers of modified sugars with short peptides
Peptidogylcan
Many bacteria have a _____ _____ outside the cell wall for extra protection
Sticky capsule
Many bacteria have _____ which act as a surface appendage
Pili
Highly compacted, NOT transcribing; visible
Heterochromatin
More open, actively transcribing; NOT visible
Euchromatin
Heterochromatin; one X chromosome in females
Barr body
Adding methyl groups to DNA bases to prevent transcription
DNA Methylation
Allow transcription to occur because the DNA is in the euchromatin form
Chromosome puffs