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30 Cards in this Set

  • Front
  • Back
Atmospheric gases that most often influence microbial growth are ? and ?
O2 and CO2
? gas has the greatest impact on microbial growth
O2(Oxygen gas)
As Oxygen enters into cellular reactions it is transformed into several toxic products ? and ?
O2-(one molecule of Oxygen by itself is a free radical)
H2O2(Hydrogen peroxide)
Most cells have developed enzymes that go about scavenging and neutralizing the free radical chemicals developed during cellular reaction and the enzymes are ? and ?
Superoxide dimutase(SOD)
and
Catalase
Cells that are ? have one or both of the chemicals required to eliminate the free radical 02- and H202 depending on how much O2 they will come in contact with
aerobic
SOD and Catalase are ? for aerobic organisms
essential
Catalase specifically breaks apart the toxic product ?
H2O2(hydrogen peroxide)
An ? can use gaseous Oxygen in its metabolism and possesses the enzymes ? and ? needed to process toxic oxygen products
aerobe
SOD(Super Oxide Dimutase)
Catalase
An ? aerobe cannot grow without oxygen
obigate
A ? aerobe does not require oxygen for its metabolism and is capable of growth in the absence of it, like the bacteria ?
facultative

E.coli
A ? does not grow at normal atmospheric concentrations of O2 but requires a small amount of it in metabolism
Microaerophile
A ? lacks the metabolic enzyme systems for using O2 in ? or ?
Anaerobe
respiration
metabolism
?/? anaerobes cannot tolerate any free O2 in the immediate environment and will die if exposed to it.
Strict
obligate
? anaerobes do not utilize oxygen but can survive and grow to a limited extent in its presence. It tolerates O2 but doesn't use it or need it
Aerotolerant
O2 is the final ? acceptor in the ETC
electron
The device used to grow anaerobic microbes is called a ? or ?
anaerobic chamber

gas chamber
The gas generator envelope has ? added to the chemicals to generate H2 and CO2 and they combine with O2 to form H20 which is visible as ? on the chamber walls
water

condensation
The anaerobic indicator strip is dipped in ? If there is O2 present it will turn ? in color. If there is no O2 present it will stay ? in color
Methylene blue

blue

white
Fluid Thioglycolate is a ? media that absorbs oxygen or slows its penetration in the medium.
reducing
Fluid Thioglycolate is used for growing ? or used for determining ? requirements
anaerobes

02
All microbes require some ? in their metabolism
CO2
? need a higher amount of CO2 than normally present in atmospheric gas
capnophiles
Proteins can be denatured by the pH of ? and ?
acids and bases
The majority of organisms live and grow in habitats between pH ? and ?
6 and 8
Acidophiles are ? to acidic environments
obligate
Proteins can handle slightly ? solutions
alkaline/basic
Soaps are ? in nature so they can break apart cell membranes of microbes
alkaline
Organisms are more likely to be adapted to ? pH environments
acidic
The organism that grows between 0 and 1 pH is ?
Euglena mutabilis
Archae that lives in hot coal piles at a pH of 1-2 is ?
Thermoplasms