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36 Cards in this Set
- Front
- Back
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What is a minimal growth agar that contains no supplements?
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TSA
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What will grow on TSA?
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Almost all bacteria
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What won't grow on TSA?
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-Corynebacteria
-Haemophilus |
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MacConkey Agar:
-Selective for -Differentiates between |
-Selective for gram neg's
-Differentiates lactose fermentors from nonfermentors |
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What color do lactose fermentors become on MAC?
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Pink
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What color do lactose nonfermentors become on MAC?
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Yellow/clear
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What are the 3 ingredients and indicator in MAC?
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-Peptones
-Deoxycholate -Lactose -Neutral red |
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When does the neutral red indicator become clear/yellow? When does it become red/magenta?
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Clear/yellow above 8.0 pH
Red/magenta below 6.8 pH |
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What are 4 bacteria that turn MAC pink and ferment lactose?
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-E. coli
-Klebsiella -Enterobacter -Citrobacter |
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What are 3 bacteria that do not turn pink and do not ferment lactose??
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-Pseudomonas
-Shigella -Salmonella |
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What gram negative rod will not grow on MAC?
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Haemophilus
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What bacteria is mannitol salt selective for?
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Halophilic - salt tolerant; usually these are gram pos only.
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What makes MS selective? What makes it differential?
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6.5% salt
Phenol red indicator |
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What color indicates mannitol fermentation? What indicates alkaline products?
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Yellow = fermentation
Pink = alkaline/nonfermentor |
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What does Blood Agar contain?
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Sheep RBCs that enrich the nutrients in the agar.
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What organisms are alpha hemolytic?
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-Strep pneumo
-Strep viridans |
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What organisms are beta hemolytic?
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-Strep A
-Strep B |
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What toxins are important for causing beta hemolysis?
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Streptolysin A (stabile)
Streptolysin O (oxygen labile) |
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What organisms are nonhemolytic (gamma)?
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-Enterococci
-Strep group D |
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What is Listeria on blood agar?
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hemolytic
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Which corynebacterium is hemolytic on BA?
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Only the pathogen - C. diphtheriae.
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What type of hemolysis does Staph aureus cause?
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You can't say - it produces four distinct hemolysins.
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What type of agar is Chocolate agar?
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Rich and nonselective.
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What makes Chocolate agar rich?
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Heme (X factor)
and NAD (V factor) |
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How do X/V factors get into the Chocolate agar?
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They are released when Blood Agar is heated for 15 min at 80 degrees.
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What can be grown on CA?
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-many organisms
-Haemophilus -Neisseria |
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What can be added to CA to inhibit the growth of gram positive bacteria?
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Vancomycin
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What is Haemophilus ID Quad plate used for?
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Distinguishing four haemophilus species
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What does quadrant 4 contain?
What grows on it? What does it allow us to see? |
RTF Casman media - all species should grow on it; allows display of hemolysis.
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Which organisms cause clearing hemolysis on quadrant 4?
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-H. haemolyticus
-H. parahaemolyticus |
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What is on quadrant 1? What should grow here?
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X factor and TSA
-nothing grows on this one |
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What is on quadrant 2? What should grow here?
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V factor only + TSA
-H. parainfluenzae -H. parahemolyticus |
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What is on quadrant 3? What should grow here?
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X and V factors + TSA
-H. influenzae -H. haemolyticus |
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How do we incubate BA and CA and the quadplate?
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In a candle jar in the 37 degree incubator.
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What does the candle jar provide?
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-increased moisture
-2-3% CO2 |
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What 3 organisms does a candle jar support growth of?
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-Streptococci
-Neisseria -Haemophilus |
