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109 Cards in this Set
- Front
- Back
what does MacConkey plate contain
|
Bile
Lactose pH indicator |
|
what does colour change of MacConkey plate indicate
|
lactose fermenter
|
|
what is the colour change of MacConkey plate
|
orange to deep pink
|
|
do all the strains of E. coli cause diarrhoea
|
no.
EHEC EPEC ETEC EIEC |
|
clinical diseases of E. coli
|
urinary tract infection
intra-abdominal infections neonatal infection |
|
how do neonatal infection of E. coli acquire
|
going though birth canal
|
|
how does intra-abdominal infection of E. coli acquire
|
after ruptured colon
|
|
where is E. coli found in human
|
normal flora of intestine
|
|
what are the lactose fermenting aerobic -ve bacilli
|
E. coli
Klebsiella Enterobacter |
|
what are the non-motile aerobic -ve bacilli
|
Klebsiella
Shigella Acinetobacter |
|
what are the glucose fermenting aerobic -ve bacilli
|
E. coli
Klebsiella Enterobacter Serratia Citrobacter Proteus Shigella Salmonella |
|
what are the glucose non-fermenting aerobin -ve bacilli
|
Pseudomonas
Acinetobacter |
|
which aerobic -ve bacilli is capsulated
|
Klebsiella
|
|
what bacteria produce red pigment colonies on colourless plate
|
Serratia marcesens
|
|
what aerobic -ve bacilli are associated with hopsital outbreaks
|
enterobacter
serratia citrobacter |
|
what aerobic -ve bacilli can develop resistance to multiple antibiotics
|
enterobacter
serratia citrobacter |
|
what bacteria can swarm on agar surface
|
Proteus
|
|
what bacteria produce ureas
|
Proteus
|
|
clinical disease of Proteus
|
urinary tract infection
renal stone |
|
why Proteus is associated with renal stone
|
- Proteus is urease +ve
- hydrolyze urea - turn the medium to alkali - crystals deposit |
|
which bacteria has only 4 species
|
Shigella
|
|
what are the species of Shigella
|
S. dysenteriae
S. flexneri S. boydii S. sonnei |
|
clinical disease of Shigella
|
Bacillary Dysentery
|
|
Sx of bacillary dysentery
|
blood and mucus in stool
|
|
transmission of bacillary dysentery
|
fecal-oral
|
|
how many serotypes are there in Salmonella
|
>2000
|
|
clinical disease of Salmonella
|
gastroenteritis
enteric fever |
|
bacteria causing enteric fever
|
S. typhi
S. paratyphi A, B, C |
|
which aerobic -ve bacilli produce green pigment on culture
|
Pseudomonas aeruginosa
|
|
which aerobic -ve bacilli is intrinsically resistant to antibiotics
|
P. aeruginosa
|
|
morphology of Acinetobacter
|
coccobacilli
|
|
where in human can Acinetobacter be found
|
skin flora
|
|
what aerobic -ve bacilli are associated with HAI
|
enterobacter
serratia citrobacter pseudomonas acinetobacter |
|
what are the vibrios species
|
Vibrio cholerae
Vibrio vulnificus Vibrio parahaemolyticus |
|
morphology of Vibrio
|
curved rod
|
|
T/F: Vibrio is motile
|
T.
motile by single flagellum |
|
oxidase state of Vibrio
|
+ve
|
|
glucose state of Vibrio
|
fermenter
|
|
differentiation of V. cholerae and V. parahaemolyticus
|
TCBS
green to yellow if sucrose fermenter |
|
which Vibrio is sucrose fermenter
|
V. cholerae
|
|
which Vibrio is sucrose non-fermenter
|
V. parahaemolyticus
|
|
O2 requirement of Vibrios
|
facultative anaerobic
|
|
catalase state of Vibrio
|
+ve
|
|
which aerobic -ve bacilli is strongly haemolytic
|
Pseudomonas
|
|
clinical disease of V. cholerae
|
cholera
|
|
transmission of V. cholerae
|
fecal-oral
|
|
clinical disease of V. parahaemolyticus
|
gastroenteritis
|
|
clinical disease of V. vulnificus
|
necrotizing fascittis
|
|
which bacteria cause necrotizing fascittis
|
V. vulnificus
|
|
morphology of Campylobacter
|
seafull shape
|
|
temperature most suitable for Campylobacter
|
42
|
|
O2 requirement of Campylobacter
|
microaerophilic
|
|
medium for Campylobacter
|
Skirrow medium
|
|
Skirrow medium for?
|
Campylobacter
|
|
appearance of Skirrow medium
|
tear-drop colonies
|
|
where in human is Campylobacter found
|
intestine
|
|
clinical disease of Campylobacter
|
gastroenteritis
|
|
what show tear-drop colonies
|
Campylobacter on Skirrow medium
|
|
oxidase state of Campylobacter
|
+ve
|
|
CO2 requirement of Campylobacter
|
10%
|
|
what is Skirrow medium
|
blood agar medium containing antibiotics
|
|
what are the common species of campylobacter causing diarrhoea
|
C. coli
C. jejuni |
|
which Campylobacter grow well at 37
|
C. fetus
|
|
morphology of Helicobacter pylori
|
S-shape
|
|
urease state of Helicobacter pylori
|
+ve
|
|
O2 requirement of Helicobacter pylori
|
microaerophilic
|
|
site of colonization of Helicobacter pylori
|
gastric mucosa
|
|
clinical disease of Helicobacter pylori
|
peptic ulcer
|
|
what bacteria colonize gastric mucosa
|
Helicobacter pylori
|
|
morphology of Haemophilus influenzae
|
coccobacilli
pleomorphic |
|
CO2 requirement of Haemophilus influenzae
|
5%
|
|
where can Haemophilus influenzae be found in human
|
URT
|
|
culture medium for Haemophilus influenzae
|
chocolate agar
|
|
what is chocolate agar
|
heated blood agar
X factor (haemin) V facter (NAD) |
|
which serotypes of Haemophilus influenzae is the most virulent
|
type b
|
|
what dose type b Haemophilus influenzae cause
|
epilottitis
|
|
which Haemophilus influenzae vaccine is available
|
Hib vaccine
|
|
which bacteria show satellitism
|
Haemophilus influenzae
|
|
what is satellitism
|
growth of Haemophilus influenzae on blood agar (X factor) near S. aureus which produce V factor
|
|
what does Legionella pneumonphilia cause
|
Legionnaire's disease
|
|
what is legionnaire's disease
|
atypical pneumonia
|
|
culture medium for legionella pneumophilia
|
BCYE agar
|
|
where can legionella pneumophilia be found
|
water
|
|
CO2 requirement of legionella pneumophilia
|
5%
|
|
culture medium for Bordetella pertussis
|
Bordet-Gengou agar
|
|
clinical disease of Bordetella pertussis
|
Pertussis
|
|
what are the animal-associated aerobic -ve bacilli
|
Brucella
Yersinia pestis |
|
transmission of Brucella to human
|
- animal-contact by cut, abrasion
- ingestion of contaminated food product |
|
Sx of Brucella
|
persistant fever
bacteremia |
|
transmission of Yersinia pestia
|
flea
|
|
clinical disease of Yersinia pestis
|
Plague
|
|
natural of Yersinia pestis
|
rodents
|
|
morophology of Neisseria
|
kidney bean shaped
diplococci |
|
CO2 requirement of Neisseria
|
5-10%
|
|
catalase state of Neisseria
|
+ve
|
|
oxidase state of Neisseria
|
+ve
|
|
colonization sites of Neisseria
|
oropharynx
GI genitourinary tract |
|
culture of Neisseria gonorrhoea
|
modified thayer martin agar
New York City agar |
|
imporant bacteria in Neisseria
|
N. gonorrhoeae
N. meningitidis |
|
what does N. gonorrhoeae ferment
|
glucose only
|
|
what does N. meningitidis ferment
|
glucose
maltose |
|
clinical disease of N. gonorrhoeae
|
gonorrhoea
neonatal conjuntivitis |
|
clinical disease of N. menngitidis
|
meningitis
bacteremia |
|
morphology of Moraxella catarrhalis
|
coccobacilli
|
|
clinical diseases of Moraxella catarrhalis
|
bronchitis
pneumonia otitis media |
|
which Neisseria shows antibiotic resistance
|
N. gonorrhoeae
|
|
what environment does Neisseria sensitive to
|
cold and dry
|
|
which Neisseria has vaccine
|
N. meningitidis
|
|
what is the normal site of Neisseria
|
oral
|