Aerobic Gram-Positive Bacilli

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Corynebacterium spp. Characteristics

Back 1

Facultatively anaerobic, gram-positive, non-spore-forming rods, club-shaped, Chinese letters, babes-ernst granules

Front 2

Corynebacterium diphtheriae virulence factors

Back 2

Exotoxins: diphtheriae toxin--> lysogenized by bacteriophage with toxin gene (tox+) toxin A&B

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Diagnostics of corynebacterium

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Aerobic conditions at 37°C

Catalase positive, non-motile, urease neg., nitrate reduction positive

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Type of agar (corynebacterium)

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Loeffler's serum agar: pleomorphism & metachromatic, Cystine-tellurite blood agar CTBA: black & gray colonies, Modified tinsdale medium: black colonies with brown halos

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Elek's test

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Immunodiffusion test: filter paper with diphtheriae antitoxin added to plate, look for specific precipitin lines

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DPT

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Diphtheriae- Pertussis-Tetanus: given to children routinely as part of immunization regimen

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Listeria monocytogenes characteristics

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Coccobacillus appears like cocci, gram-positive, nonsporulating forming rods

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Listeria monocytogenes virulence factors

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Hemolysin: listeriolysin O, Catalase, surface protein p60, phospholipase C, and superoxide dismutase

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L. monocytogenes diagnostic

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SBA, chocolate agar, prefers slightly increased CO2, colonies: narrow zone of hemolysis, small round, smooth and translucent. Optimal growth: 30°- 35°C

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Identifying L. monocytogenes

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Catalase positive, motility: "umbrella", CAMP test: block type hemolysis, Hippurate hydrolysis positive, Bile Esculin positive

Hint 10

Front 11

Erysipelothrix rhusiopahie

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Thin, Gram-positive, non-spore-forming, pleomorphic rods

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Diagnostics of E. Rhusioparhiae

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Culture: CO2 needed, Blood agar or chocolate agar: colonies are grey or translucent, alpha hemolysis.

*production of hydrogen sulfide on triple sugar iron