Adv Micro Study Test 2

Front 1

Describe MALDI-ToF system as it applies to bacterial identification

Back 1

Molecular weight and quantities of protein molecules

Front 2

Malachite green

Back 2

What constituent of Lowenstein-Jensen (LJ) medium is added to suppress the growth of gram positives?

Front 3

RFLP Restriction Fragment Length Polymorphism

Back 3

Determine if multiple isolates from various hospital units were related, which method would you use?

Front 4

nonchromogens

Back 4

species whose colonies are a buff color and exposure to light does not induce pigment formation

Front 5

The proteins detected by the MALDI-ToF system are generally in what molecular weight range?

Back 5

2,000 to 20,000 Daltons

Front 6

Known to cause inflammatory bowel disease referred to as Johne's disease in cattle, sheep, and goats, this organism has been isolated from the bowel mucosa of patients with Crohn's disease.

Back 6

M. paratuberculosis

Front 7

The prefered basic nonselective media used for the isolation of Mycobacterium tuberculosis is an egg-based medium known as

Back 7

Lowenstein-Jensen

Front 8

Infections with species of Mycobacterium other than tuberculosis

Back 8

does NOT lead to disease with M. tuberculosis

Front 9

The PNA-FISH test is designed to detect

Back 9

bacterial nucleic acid targets

Front 10

In a hybridization reaction, the pH affects

Back 10

the stability of double-stranded nucleic acid molecules in solution

Front 11

M. kansasii reactions

Back 11

Semi Q Catalase POS
Nitrate reduction POS
Tween hydrolysis POS

Front 12

Genetic probes are commercially available for use in identifying M. tuberculosis, avium and intracellulare

Back 12

True

Front 13

The mycobacterial species that occur in humans and belong to the M. tuberculosis complex include

Back 13

M. tuberculosis
M. bovis
M. bovis BCG
M. africanum

Front 14

The target nucleic acid strand is

Back 14

the DNA or RNA sequence unique to the organism of interest

Front 15

How long are mycobacterial cultures routinely held?

Back 15

6-8 weeks

Front 16

Tween 80 test

Back 16

lipase that is able to hydrolyze polyxyethylene sorbitan monooleate into oleic acid and polyoxyethylated sorbitol

Front 17

Runyon system of classification

Back 17

growth rate and colonial pigmentation

Front 18

tube coagulase relies on what characteristics to identify Gram-positive cocci

Back 18

phenotypic

Front 19

What component of mycobacteria is used for identification in the nucleic acid hybridization assays?

Back 19

Ribosomal RNA

Front 20

PNA in PNA-FISH stands for what

Back 20

Peptide Nucleic Acid

Front 21

An infection caused by M. leprae is typically diagnosed by

Back 21

a positive acid fast smear

Front 22

M. tuberculosis

Back 22

LJ growth of buff colored and rough colonies after 4 weeks in culture. Nitrate POS.

Front 23

Two of the most important advantages of NAAT systems

Back 23

Higher sensitivity and faster TAT

Front 24

CDC does NOT recommend the following drug for the treatment of M. tuberculosis

Back 24

ciprofloxacin

Front 25

7H10 medium

Back 25

suggested medium for the cultivation of Mycobacterium tuberculosis that is an agar-based medium

Front 26

FISH technique allows for

Back 26

whole cell visualization of the target pathogen(s)

Front 27

M. fortuitum complex

Back 27

negative AFB
culture growth at <7 days on LJ that stains as AFB