Abac Biol 2050 Lab Midterm (Branch)

Front 1

Just after decolorizer is applied acid-fast bacteria will appear ___ and non-acid bacteria will appear ______.

Back 1

Fushcia and colorless

Front 2

After brilliant green is applied as counter stain, acid-fast bacteria will appear _____ while non-acid bacteria appear _____.

Back 2

Fuschia and green

Front 3

Mycobacterium is acid-fast ___ (positive/negative) and ____ shaped

Back 3

Positive

bacillus

Front 4

Appearance of Staphylococcus aureus after acid-fast stain

Back 4

Clusters of green cocci (acid-fast negative)

Front 5

E. coli is gram ____ (negative/positive)

Back 5

positive

Front 6

Most critical step of the gram stain

Back 6

Decolorization

Front 7

If you forgot to counter stain at the end of a gram-stain procedure, gram negative would appear ______ and gram positive would appear _______

Back 7

Colorless and purple

Front 8

Microscopic morphology and Gram reaction for Staphylococcus aureus

Back 8

gram-positive staphylococci

Front 9

Why is it important crystal violet be contrasting color to safranin

Back 9

Difference in color between the two stains allows you to differentiate between gram negative and gram positive cells by observing the color of organism

Front 10

Microscopic morphology and Gram reaction for Micrococcus luteus

Back 10

gram-positive staphylococci

Front 11

Purpose of allowing bacteria smear to dry when placed on slide

Back 11

Drying helps remove excess water to ensure optimal heat fixation and true arrangements are seen

Front 12

Clusters of spherically shaped are called___

Back 12

staphylococci

Front 13

Chains of rod-shaped are called ____

Back 13

Streptobacilli

Front 14

Decolorizing agent in spore stain is _____

Back 14

Water

Front 15

Name two acid-fast organism.

Back 15

Mycobacterium

Nocardia

Front 16

______ bacteria retain carbol fuchsin after acid-alcohol, ______ are decolorized

Back 16

Acid-fast

non-acid fast

Front 17

The bacilli in this stain are gram _____

Back 17

negative (pink)

Front 18

These cocci are gram _____

Back 18

positive (purple)

Front 19

These bacteria are acid-fast _____

Back 19

positive (fuchsia)

Front 20

Do these bacteria contain endospores?

Back 20

Yes, Endospore positive (pink with green)

Front 21

These bacteria are acid-fast _____

Back 21

negative (bluish-green)

Front 22

Do these bacteria contain endospores?

Back 22

Endospore negative (pink) no green endospores

Front 23

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 23

Escherichia coli
beige, mucoid, glistening, opaque, medium convex, round, entire

Front 24

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 24

Pseudomonas
Medium, opaque, circular, serrated, irredescent, beige with green pigment
Smells like grapes

Front 25

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 25

1. Serratia

2. Red, medium, irregular, circular, umbinate, opaque

Front 26

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 26

1. Micrococcus
2. Bright buttery yellow, smaller, round, opaque, entire, convex, creamy

Front 27

Fomite

Back 27

Object or substance capable of carrying infectious organisms

Front 28

Aseptically

Back 28

Performed under sterile conditions

Front 29

Density

Back 29

Opaque, transparent, or translucent

Front 30

Consistency

Back 30

Butyrous, mucoid, dull, dry, or powdery

Front 31

Texture

Back 31

Smooth, rough, wrinkled, glistening, or irredescent

Front 32

Serial dilution

Back 32

Stepwise dilution of substance in solution

Front 33

Colony forming unit- CFU

Back 33

Viable bacteria; colony

Front 34

TFTC

Back 34

Too few to count

Front 35

TNTC

Back 35

Too numerous to count

Front 36

Nosocomial infection

Back 36

Infection development favored by a hospital environment

Front 37

Gram stain reagents, time, and purpose

Back 37

1. Crystal violet (primary stain)- 60 sec

2. Gram's iodine (mordant)-60 sec.

3. ethyl alcohol (decolorizer) 10-15 sec

4. safranin (counter stain) 30-60 sec

Front 38

Acid-fast reagents, purpose, and time

Back 38

1. Carbol fuchsin (primary stain) 5 min

2. acid-alcohol (decolorizer) 30 sec

3. brilliant green (counter stain) 2 min

Front 39

Endospore stain reagents and time

Back 39

1. Malachite green (endospore stain)-steam 5 min

2. safranin (vegetative stain)- 1 min

Front 40

1. Name the organism we have seen in lab that could have been used to make this slide.

2. Describe the microscopic morphology

Back 40

1. Bacillus

2. Gram positive streptobacilli

Front 41

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 41

1. Mycobacterium

2. small, dry, pale yellow, rough

Front 42

These bacteria are acid-fast _____

Back 42

negative (blue-green)

Front 43

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 43

1. Bacillus
2. Beige, dry, rough, opaque, large and flat

Front 44

1. The genus of this organism is ____

2. Describe the colonial morphology.

Back 44

1. Staphylococcus aureus

2. medium, opaque, golden, round, entire, convex, smooth

Front 45

1. Name the 3 organisms we have seen in lab that could have been used to make this slide.

2. Describe the microscopic morphology

Back 45

1. Serratia, E. coli and Pseudomonas
2. Gram negative bacillus

Front 46

1. Name the organisms we have seen in lab that could have been used to make this slide.

2. Describe the microscopic morphology

Back 46

1. Staphylococcus aureus

Staphylococcus epidermidis

2. Gram positive staphylococci

Front 47

Escherichia coli-

microscopic morphology _____
Gram _____
Acid-fast ____

Endospore _____

Back 47

bacillus

gram negative

acid-fast negative

endospore negative

Front 48

Staphylococcus aureus-

microscopic morphology ______
Gram _____
Acid-fast ____

Endospore _____

Back 48

staphylococci

gram positive

acid-fast negative

endospore negative

Front 49

Micrococcus luteus-

microscopic morphology _____
Gram _____
Acid-fast ____

Endospore _____

Back 49

cocci in tetrads

gram positive

acid-fast negative

endospore negative

Front 50

Bacillus subtilis-

microscopic morphology ______
Gram _____
Acid-fast ____

Endospore _____

Back 50

streptobacilli

gram positive

acid-fast negative

endospore positive

Front 51

Serratia-

microscopic morphology _____
Gram _____
Acid-fast ____

Endospore _____

Back 51

bacillus

gram negative

acid-fast negative

endospore negative

Front 52

Pseudomonas aerugenosa-

microscopic morphology ______
Gram _____
Acid-fast ____

Endospore _____

Back 52

bacillus

gram negative

acid-fast negative

endospore negative

Front 53

Mycobacterium-

microscopic morphology ______
Gram _____
Acid-fast ____

Endospore _____

Back 53

bacillus

gram positive

acid-fast positive

endospore negative

Front 54

1. Name the organism we have seen in lab that could have been used to make this slide.

2. Describe the microscopic morphology

Back 54

1. Micrococcus

2. Gram positive tetrads

Front 55

Staphylococcus epidermidis-

microscopic morphology ______

Gram _____

Acid-fast ____

Endospore _____

Back 55

staphylococci

gram positive

acid-fast negative

endospore negative

Front 56

1. The genus and species of this organism is ____

2. Describe the colonial morphology.

Back 56

1. Staphylococcus epidermidis

2. medium, round, opaque, flat, entire, white, creamy