2638L Fall 2015 Rotation 4

Front 1

In the feline there are 2 types of retics, the punctate and the aggregate. Which of these types do you count?

Back 1

Aggregate

Front 2

What is the purpose of performing a reticulocyte count?

Back 2

Access response of the bone marrow to the anemia

Front 3

Which species of animal does not have circulating retics?

Back 3

Horse

Front 4

How many retics are seen in this picture of dog blood?

Back 4

3

Front 5

What stain is used in preparing a reticulocyte count?

Back 5

New Methlyene Blue, NMB

Front 6

What would a retic be called under diff-quik stain?

Back 6

Macrocytic polychromatic rbc

Front 7

Which retic is considered the younger kind, aggregate or punctate?

Back 7

Aggregate

Front 8

How many RBC's should be in a each field when looking for retics?

Back 8

100 RBC

Front 9

What is the observed retic count?

Back 9

The observed retic count is the number of retics per 1000 RBC's

Front 10

What is the formula for the corrected retic count?

Back 10

Patient PCV / normal PCV X observed retic

Front 11

What is the formula for the absolute retic count?

Back 11

Take the observed retic (before you change it to a percentage) and multiply it times the total RBC count.

Front 12

What is the procedure for retic staining, incubated/dry?

Back 12

Procedure: incubate equal parts blood/NMB at room temp for 15 min. Make thick push smear (since diluted and usually anemic). Air dry and evaluate on oil immersion.

Front 13

Aggregate retics have less than 5 clumps of reticulum. True or False?

Back 13

False. Aggregate retics have greater than 5 clumps of reticulum.

Front 14

What is the procedure for NMB wet-prep?

Back 14

Procedure: Take dry coverslip unstained smear and place bloodside down onto drop of NMB on slide.

Front 15

Why is the incubated method the preferred method over the wet-prep method?

Back 15

Incubated method preferred since difficult to distinguish punctate from aggregate, and harder to read.

Front 16

Punctuate retics have less than 5 clumps of reticulum. True or False?

Back 16

True

Front 17

The wet prep method is used when?

Back 17

A limited amount of blood available.

Front 18

What kind of retics are these?

Back 18

Aggregate

Front 19

Your dog has a 15% PCV, and you counted 76 retics. What would be the corrected retic count for this animal?

Back 19

2.5%

Front 20

What happens when retics are stained with NMB?

Back 20

When stained with NMB the ribosomes and other organelles of the immature RBC clump into visible granules.

Front 21

Define a reticulocyte.

Back 21

A reticulocyte are immature erythrocytes that contain organelles that are lost as the cells mature. These organelles account for the diffuse blue-gray or polychromatophilic staining of the immature cells with Wright’s stain.

Front 22

What is considered regenerative in regard to the absolute retic count?

Back 22

In regard to the absolute rectic count the normal dog and cat the absolute retic count is >60,000/µl. In the anemic animal one would like to see an absolute count of 100,000/µl or higher.

Front 23

Define reticulocyte count.

Back 23

A reticulocyte count is an expression of the percentage of RBC's that are reticulocytes.

Front 24

Feline reticulocytes come in 2 varieties:

Back 24

punctate and aggregate

Front 25

Canine reticulocytes are almost always

Back 25

aggregate

Front 26

The retic count is performed on oil but a suitable viewing area is found on high power.True or False?

Back 26

True

Front 27

Would this be considered a proper area to count your retics?

Back 27

Yes

Front 28

When counting retics, you view ________ number of fields with ___________ number of retics to get a total count of ________.

Back 28

10, 100, 1000

Front 29

In cases of severe blood loss anemia, how long might it take for retics to be seen in blood?

Back 29

2-4 days

Front 30

Why might you not see retics in the blood until a few days after an anemic patient is admitted?

Back 30

The blood may not start regenerating immediately and may take a few days.