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20 Cards in this Set
- Front
- Back
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Define a reticulocyte
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Reticulocytes are young RBCs specifically stained with a supravital stain, such as New Methylene Blue, in order to enhance their remaining nucleolar detail. This detail consists of organelles which have not yet been pushed out of the cell.
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Which domestic species does not have reticulocytes in the peripheral blood?
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Equine
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How is the reticulocyte observed count obtained?
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Number of retic counted/1,000 cells observed
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How is the reticulocyte corrected count obtained?
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(Patient PCV / normal PCV) x Observed retic %
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How is a absolute Retic count obtained?
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Retics counted/ 1000 x RBC count
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What is considered range of absolute count is considered regenerative?
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80,000-100,000mm3
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Which reticulocytes are counted, aggregate or punctate?
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Aggregate
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How many fields should be counted during a reticulocyte count?
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10; retic count observed per 1000 rbcs
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How is a Reticulocyte stain made?
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Incubate equal parts of blood and NMB at room temp for 15 mins, then make a push smear, Air dry and evaluate on oil immersion
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Why are reticulocyte counts important to assess?
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Gives us information on bone marrow's response to anemia
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What is the traditional stain for counting reticulocytes?
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New Methlyene Blue
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What type of reticulocytes have non-clumping reticulum of 1-4 dots?
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Punctate
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What type of reticulocytes have large clumps of retuculum and often appear bigger and bluer than surrounding RBCs?
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Aggregate
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Are reticulocytes are efficient at utilizing oxygen?
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No
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What is another name for a reticulocyte?
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An immature RBC
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If an animal had an absolute retic of 90,000, would it indicate regenerative or non-regenerative anemia?
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Regenerative
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If an animal had an absolute retic of 1,000, would it indicate regenerative or non-regenerative anemia?
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Non-regenerative
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Obtain the corrected reticulocyte count:
Patient: Canine Patient PCV: 37% Normal Canine PCV: 45% Observed retic count: 2% |
(37% / 45%) x 2% = 1.64
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Obtain the corrected reticulocyte count:
Patient: Feline Patient PCV: 28% Normal Feline PCV: 37% Observed retic count: 2% |
(28% x 37%) x 2% = 1.51
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Obtain observed reticulocyte count:
20 retics observed out of 1000 rbcs |
20/1000= 0.020 or 2.0%
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