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20 Cards in this Set
- Front
- Back
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What preferred stain is used to count retics?
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NMB stain
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Why is reticulocyte counting useful?
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The reticulocyte count is the preferred method for quantitating a regenerative response.
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How do you know if you’re seeing reticulocytes in a feline blood smear?
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When you see both aggregate and punctate reticulocytes.
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Canine reticulocytes are almost always puntate. True or false?
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False.
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When stained with NMB, what is shown on the reticulocytes?
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When stained with NMB the ribosomes and other organelles of the immature RBC clump into visible granules.
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Reticulocytes are what?
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Immature red blood cells
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Which domestic species does not have reticulocytes in the peripheral blood?
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Horses.
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Write down the normal dog and cat absolute retic count.
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In the normal dog and cat the absolute retic count is >60,000/µl.
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How is the reticulocyte percentage obtained (observed AND corrected)?
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The reticulocyte percentage is adjusted by the ratio of the observed hematocrit
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30 blood cells in a certain field is a good place to count retics. True or false?
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True.
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20. 80,000 - 100,000 mm3 is considered regenerative or nonregenerative?
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Regenerative
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Describe the shape of an aggregated reticulocyte.
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Contain large clumps of retuculum and often appear bigger and bluer than the RBC's around them.
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If stained with diff quick instead of NMB, how the blood cells appear?
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The immature RBC appears polychromataphillic on the Dif Quick Smear (big-macrocytic and blue).
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How many types of reticulocytes do felines have?
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2 types: aggregate and punctate
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Reticulocytes are useful in detecting what?
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Regeneration and response to therapy.
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Name a disease that deals with reticulocytes.
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Anemia, bone marrow depression, blood loss, e.t.c
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Explain the wet method of making a retic slide.
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The coverslip smear (unstained) is dropped blood side down into a drop of NMB on a slide. This is left undisturbed for 15 minutes and then observed for reticulocytes. This slide prep is much more difficult to interpret for retics.
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Which retic in cats is counted?
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Agggrate.
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Which is seen more often: hemolytic or hemorrhagic anemia
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Hemolytic
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What are the Ribosomes?
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Ribobucleoprotein particles concerned with protein synthesis
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