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18 Cards in this Set
- Front
- Back
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Pour plating |
Sample combined with molten agar, grows in and on |
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Spread plate |
Sample spread on surface, grows on |
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Filtration |
Method of counting |
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MPN test |
When sample can't be grown in solid agar, number of tubes with growth are counted and use a chart |
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MPN uses |
Only broth or when growing large amounts |
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Direct microscopic counts |
Very fast, uses a cytometer, is not very accurate |
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Indirect methods of growth |
Turbidity, metabolic activity, dry weight |
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Turbidity |
Won't lose the sample, measures optical density |
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Metabolic activity |
Production of CO2, or acid |
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Dry weight |
useful for looking at growth of increasing size of cells, sporulation, production of capsules |
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Snap freezing |
Add antifreeze which prevents large ice crystals |
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Lyophilization |
Lasts longer than 10 years |
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Sublimination |
No pressure present, gas to solid, solid to gas |
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EPS |
Provides protection, covers whole community |
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1886 mayer |
Tobacco disease |
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1892 iwanoski |
Found an infectious agent that can pass through a filter |
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Why are viruses not alive |
They cannot replicate on their own |
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Morphology |
Helical, polyhedral, enveloped, complex |
