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26 Cards in this Set
- Front
- Back
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Enteric bacteria |
Gram negative bacteria that inhabit intestines as normal flora |
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SCA |
Used to differentiate gram negative bacteria on the basis of citric acid utilization |
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SCA color agent |
Bromthymol blue |
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SIM abbreviation |
Sulfide Indole Motility |
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SIM |
Used to distinguish enteric bacilli on the basis of H2S production, indole formation, and motility |
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SIM distinguishes between |
Salmonella and Shigella genera |
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SCA distinguishes between |
E. Coli and E. Aerogenes |
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Indole is a byproduct of |
Tryptophan and tryptophanase |
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Organisms that are positive for sulfide production |
Enzyme cysteine desulfurase, makes black ppt |
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TSI |
Used to distinguish enteric bacteria based on their ability to ferment glucose, lactose, and/or sucrose |
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If TSI is red in the slant |
If lactose and/or sucrose cannot be fermented |
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TSI: ferments glucose initially |
the pH is lowered and the slant and butt turn yellow |
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TSI: if lactose and/or sucrose is also fermented |
the pH is lowered and the slant and butt turn yellow |
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TSI: if none is fermented |
Peptone will be broken down and the slant will be red |
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Urease test |
Tests an organisms ability to produce urease |
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Urease is a what |
Exoenzyme that hydrolyzes urea to ammonia and CO2 |
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Phenol red does what |
Turns yellow in an acidic environment, if in an alkaline environment it turns pink |
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Rapid urease-positive organisms |
Enterics that can degrade urea rapidly, genus proteus |
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Chromagar |
Helps differentiate enterobacteriaceae based on the color of the colony |
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What is chromagar commonly used for |
Distinguishes types of gram negative UTIs |
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Gram staining |
Gram (+) = thick peptidoglycan = purple Gram (-) = thin peptidoglycan = pink |
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Simple stain |
Using a single stain to color bacteria |
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Chromophore |
Colored part of the stain |
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Basic stain |
Positive charge |
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Acid stain |
Negative charge |
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Gram stain procedure |
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