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15 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Protoplasmic activities Note : fresh tissue are not permanent |
*motion *mitosis *phagocytosis *pinocytosis |
M2 P2 |
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Methods of fresh tissue preparation |
*teasing /dissociation *spreading *smear prepration (two slide mtd.) *frozen section |
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Four types of smear preparation / two slide method |
*streaking *spreading *pull -apart * touch preparation / impression smear |
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More commonly used method of Freezing Note : slow freeze cause distortion of tissue due to crystals artifacts |
*liquid nitrogen *isopentane cooled by liquid nitrogen *carbon dioxide gas *aerosol spray |
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Process where tissue specimen is selected and immersed in Isotonic salt Note: phase constrast or brightfield microscopy for unstained. Differential dye for stained |
Teasing or dissociation |
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Process where small pieces of tissue not more than 1mm are placed in slide and compressed with another slide |
Squash preparation or crushing |
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Process of examining sediments or sections where cellular materials are spread over a slude by means of wireloop / applicator Note : useful for cytological examinations especially cancer diagnosis |
Smear preparation (two slude method) |
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Use of applicator stick or platinum loop where materials applied in zigazag line thruout the slide Note : avoid too thin and too thick |
Streaking |
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A selected portion of materials is transferred to clean slide and spread moderately thick film by teasing the mucous strands with applicator rick Note : recommended for fresh sputum,bronchial aspirates, thick mucoid secretions |
Spreading |
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Done by placing a drop of secretion or sediment upon one slide and facing to another clean slide Note : useful for serous fluid , conc. Sputum, enzymatic lavage samples from gastrointestinal tract and blood smear |
Pull apart |
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Special method for smear preparation. The surface of the clean glass slide Note: they can eamined the cells without deatroying their actual intercellular relationship |
Touch preparation / impresion smear |
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Tjis metjod is utilze when rapid diagnosis of tissue in question is required Note : recommended when nervous tisaue and lipids asr demostrate |
Frozen section |
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What is the thickness cut from fresh tissue frozen on microtome with CO2 or Cryostat. |
10 to 15 um |
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What is the required atmosphere to kept in cold chamber |
-10 to -20°C |
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Applications in histochemical for both fixed or unfixed are commonly used for: |
* rapid pathologic diagnosis during surgery *diagnostic and research enzyme histochemistry *diagnostic and research demonstation of soluble substances such as lipids and carbohydrates * immunofluorescent and immunohistochemical staining *some specialized silver stains , esp. Neuropathology |
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