Lab Five: Microscope and Simple Staining
Introduction
A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a scale or ruler, is flat circle of glass upon which are etched in equally spaced division. When placed in the eyepiece the line is superimposed may be calibrated by using a stage micrometer on which parallel lines are exactly 10um apart etched. By determining …show more content…
It was used to measure the size of objects. For measurement with greater accuracy, it use together with a stage micrometer. When combined with a precision stage micrometer, the standard eyepiece reticle provides a rapid, convenient, and accurate means of conducting measurement in the microscope. The use of a stage micrometer was to calibrate the ocular micrometer. A stage micrometer is essentially a ruler that is mounted to the microscope slide that does have units (millimeter or micrometer).
When calibrating, the stage micrometer was line up with the ocular micrometer and counted the number of division on the ocular micrometer per millimeter or micrometer on the staged micrometer. As the magnification changes, the number of division will also change.
The simple staining protocol was done to achieve several objectives: to provide an appropriate contrasting background so that an object can be observed with relative ease. Most of the objects, such as bacteria are transparent and light can pass through them. This bacterium was stained, the light was reflected and the bacteria were easily visualized. Simple staining was useful to study the morphology of the bacteria, the size of the bacteria, and the arrangement of the bacteria. Only one type of dye was use and that was Methylene
Introduction
A microscope is a tool to examine enlarges image of small objects that are hard to study with the naked eyes. The light microscope, which was used in this lab experiment, is an instrument with two lenses and various knobs to focus the image. We will learn about the proper way to use and handle the microscope. Microorganisms are measured with an ocular micrometer, which is inserted into one of the microscope eyepiece. Micrometer serves as a scale or ruler, is flat circle of glass upon which are etched in equally spaced division. When placed in the eyepiece the line is superimposed may be calibrated by using a stage micrometer on which parallel lines are exactly 10um apart etched. By determining …show more content…
It was used to measure the size of objects. For measurement with greater accuracy, it use together with a stage micrometer. When combined with a precision stage micrometer, the standard eyepiece reticle provides a rapid, convenient, and accurate means of conducting measurement in the microscope. The use of a stage micrometer was to calibrate the ocular micrometer. A stage micrometer is essentially a ruler that is mounted to the microscope slide that does have units (millimeter or micrometer).
When calibrating, the stage micrometer was line up with the ocular micrometer and counted the number of division on the ocular micrometer per millimeter or micrometer on the staged micrometer. As the magnification changes, the number of division will also change.
The simple staining protocol was done to achieve several objectives: to provide an appropriate contrasting background so that an object can be observed with relative ease. Most of the objects, such as bacteria are transparent and light can pass through them. This bacterium was stained, the light was reflected and the bacteria were easily visualized. Simple staining was useful to study the morphology of the bacteria, the size of the bacteria, and the arrangement of the bacteria. Only one type of dye was use and that was Methylene