Essay on Lactase Laboratory Report

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Lab #6: Specificity of Enzyme
Lactase Laboratory Report Introduction

Enzymes are biomolecules that catalyze or assist chemical reactions. ("Enzyme Information - Disabled World", n.d.,) Without enzymes it would be impossible for an organism to carry out chemical reactions. Enzymes are proteins that carry a chemical reaction for a specific substance or nutrient. For example, the digestive enzymes help food to be broken down so it can be absorbed. Enzymes can either initiate the reaction or speed it up. Substrates are the chemicals that are transformed by enzymes. (Gunsch & Foster, 2014) Reactants are the chemicals in the absence of enzymes. Metabolic pathways that occur in a cell are determined by a set of enzymes which are
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In the first experiment we made and tested 4 solutions for glucose: milk and lactase (A), milk and water (B), sucrose and lactase (C), and sucrose and water (D). For the first part of the second experiment, we put a solution of milk and lactase in temperatures of 4°C, 25°C and 100°C. During the second part, we added a pH 4 lactase, pH7 lactase and pH 10 lactase into a test tube of milk. Both solutions were tested for glucose. Given the background, I hypothesized in experiment 1 if lactase was not be able to break down sucrose because of its shape then the presence of glucose in the treatments without milk will be low to none because glucose was either broken down by the enzyme or not present. Through experiment 1, we found that the test results of the treatments that contained milk yielded a higher amount of glucose and the treatments with glucose had very little glucose. In experiment 2, I hypothesized that a low or neutral pH will give a higher result for the glucose test and predicted that 25°C would be the best for this specific enzyme. The result was that a pH of 7 and 4 contained a higher amount of glucose and room temperature was the best for lactase efficiency. Materials and Methods
Lactase Enzyme Specificity
Procedure 1 – Scientific Method
After reading the directions for procedure

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