Spirulina Cell Experiment Hypothesis

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3.0 Hypothesis
I assume that as the solution is treated with higher amount of fertilizer, the Spirulina cells will reproduce better and survive for longer time as there will be higher amount of nutrients supplied. Therefore, it is assumed that the Spirulina-concentration of the trials treated with 1.0mL fertilizer will be higher than those treated with 0.5mL or 0.0mL. Although wastewaters contain elements which are vital for the Spirulina growth, as it contain other unknown substances as well, its influence on the rate of growth of the Spirulina's cannot be unambiguously hypothesized. Since bicarbonate and sodium carbonate were not added to the effluent water but only to the portable water, it is assumed that the higher concentration of the
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It is true that lower pH enables higher competition and it is interesting to check its effects, however, at the end of the experiment, a microscopic observation showed that no other species existed in the mediums. This means that higher competition was not the problem, but it is possible that the lower pH caused pH- enzyme activity- photosynthesis???
• In order to make this report a reliable and honest source, uncertainty values of the volumes and absorption are mentioned (will be mentioned).
• Before each measurement I have checked carefully that the cuvette is clean and dry so when the wavelength passes in the spectrophotometer, the absorption results will not get distorted.
• I have noticed that in some of the samples there were Spirulina cells that have accumulated to a visible strains, darker than the Spirulina which is more spread in the water of the sample. Hence, before placing the cuvette in the spectrophotometer it is recommended to mixe its content by closing the opening with a clean finger and shaking it so the solution will get as homogeneous (uniform) as possible.  Controlled

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