The bla gene codes for beta lactamase which promotes antibiotic resistance for drugs classified as being a part of the penicillin family. In the pGLO plasmid there is a unique gene regulation system which controls the transcription of the green fluorescent protein. In order for the GFP to be expressed, arabinose sugar must be present. Arabinose sugar reacts with the AraC protein, and this reaction initiates transcription of the GFP gene. The purpose of this experiment is to see whether bacteria will be successfully transformed to express the green fluorescent protein. The hypothesis for this experiment is that in the presence of an arabinose sugar, a GFP protein will be produced, and as a result bacterial transformation will occur. Genetic transformation plays an important and beneficial role in our society. For example, in agriculture genetic transformation can result in genetic traits that prevent plants from being affected by factors such as frost and pests. Additionally, genetic transformation has a significant role in medicine. Gene therapy is now being used to treat diseases that are a result of genes functioning
The bla gene codes for beta lactamase which promotes antibiotic resistance for drugs classified as being a part of the penicillin family. In the pGLO plasmid there is a unique gene regulation system which controls the transcription of the green fluorescent protein. In order for the GFP to be expressed, arabinose sugar must be present. Arabinose sugar reacts with the AraC protein, and this reaction initiates transcription of the GFP gene. The purpose of this experiment is to see whether bacteria will be successfully transformed to express the green fluorescent protein. The hypothesis for this experiment is that in the presence of an arabinose sugar, a GFP protein will be produced, and as a result bacterial transformation will occur. Genetic transformation plays an important and beneficial role in our society. For example, in agriculture genetic transformation can result in genetic traits that prevent plants from being affected by factors such as frost and pests. Additionally, genetic transformation has a significant role in medicine. Gene therapy is now being used to treat diseases that are a result of genes functioning