The rate of respiration of yeast, using two saccharides and an amino acid, in combination with the cofactor of magnesium, was determined using respirometers. A positive control sample of yeast and water, and a negative control sample of glucose, sodium fluoride and yeast were also employed. After seventy five minutes, the sample containing glucose, magnesium and yeast showed a markedly higher production of CO2 than the other samples. This result supported the hypothesis that glucose is the most effective fuel for cellular respiration.
Introduction
Cellular respiration breaks down glucose (or other energy rich organic compounds) with the use of oxygen to obtain energy in the useable form of adenosine three phosphate (ATP), and produces carbon …show more content…
Respiration can take place with or without oxygen, however, in an anaerobic environment, fermentation occurs after glycolysis, and the reaction does not continue through the Krebs Cycle and ETC to produce ATP. Because CO2 is necessary for the production of ATP, this experiment measured CO2 production as an indicator of the presence of ATP.
Carbon dioxide, the product of respiration, was produced in varying amounts by the different samples in the experiment. The results of this experiment supported the hypothesis that glucose is the most effective fuel for cellular respiration. The respirometers containing the saccharides produced a larger volume of CO2 than the respirometers containing the amino acid or either of the controls. Graph 1 clearly shows that higher rates of respiration that were achieved using the saccharides. Graph 2 shows the negative effect that the introduction of the inhibitor, NaF, had on the reaction. Sodium fluoride acts to deplete the cells of the cofactor, magnesium, without which the cells cannot carry out the formation of pyruvates during
Introduction
Cellular respiration breaks down glucose (or other energy rich organic compounds) with the use of oxygen to obtain energy in the useable form of adenosine three phosphate (ATP), and produces carbon …show more content…
Respiration can take place with or without oxygen, however, in an anaerobic environment, fermentation occurs after glycolysis, and the reaction does not continue through the Krebs Cycle and ETC to produce ATP. Because CO2 is necessary for the production of ATP, this experiment measured CO2 production as an indicator of the presence of ATP.
Carbon dioxide, the product of respiration, was produced in varying amounts by the different samples in the experiment. The results of this experiment supported the hypothesis that glucose is the most effective fuel for cellular respiration. The respirometers containing the saccharides produced a larger volume of CO2 than the respirometers containing the amino acid or either of the controls. Graph 1 clearly shows that higher rates of respiration that were achieved using the saccharides. Graph 2 shows the negative effect that the introduction of the inhibitor, NaF, had on the reaction. Sodium fluoride acts to deplete the cells of the cofactor, magnesium, without which the cells cannot carry out the formation of pyruvates during