Wolff-Parkinson-White (WPW) syndrome is a hereditary disease. WPW syndrome causes chronic heart disease which is associated with ventricular pre-excitation and a thicker ventricle muscle. Patients with the disease have a high rate of more than 100 heart beats per minute, while a healthy person having a normal heart has 72 heart beats per minute. This increased heart beats and other factors associated with the disease result in severe cardiac disease and only heart transplantation may cure the disease.
WPW syndrome is caused by mutation in the PRKAG2 gene which changes the protein structure of γ2subunit of AMP-activated protein kinase (AMPK). AMPK is an enzyme (biological catalyst) that helps to maintain the internal energy of a cell. AMPK is made up of three subunits and the three subunits altogether help it to maintain its function inside a cell. The γ2 subunit (one of the …show more content…
AAV9 is reliable as it affects the human cardiac cells without any pathogenic effect and therefore, they are used commonly to insert genes into cells. Cas9 or CRISPR associated protein is a protein that acts as the molecular scissor and cut the DNA in the desired site and thereby editing the gene. The DNA is not withered away rather it gets repair by our cells. During the repairing process some nuclear base are added (inserted) or deleted or gets exchange with a nuclear base that suddenly ends a protein translation. This insertion and deletion is known as indel and the measurement of indel is known as indel