Winogradsky Column Discription Essay

1135 Words Oct 22nd, 2012 5 Pages
Winogradsky column description
The experiment of Winogradsky column is to observe microbiology interaction of microbes using pond mud. The aim of this paper is to describe structural and chemical features in Winogradsky column and to show the acknowledgement of microbial cells in Winogradsky column.

Materials of experiment
Pond mud
Calcium sulfate
Calcium carbonate
Shredded paper
Pond water
Aluminum foil

Methods 1. Prepare pond mud 2. Add calcium sulfate and calcium carbonate 3. Add piece of paper 4. Add pond water 5. Seal top of the column 6. Put beside window

Process (sequence of the reaction)
The less of oxygen makes oxygen enrichment at the top as well as anaerobic
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Rhodomicrobium) grow. In the absence of O2 and in the presence of light, purple nonsulfur bacteria carry out photosynthesis much like the purple sulfur do. However, when they fix CO2 to produce organic molecules, they use organic acids or ethanol as electron donors rather than H2S.
At the interface of sulfide –containing mud and the more aerobic pond water, bacteria grow that can oxidize sulfur. An example is the filamentous bacterium Beggiatoa. In one metabolic strategy for survival, Beggiatoa uses H2S as an energy source and oxidizes it to sulphuric acid. The energy released by this process is used to fix carbon and produce organic molecules. (Perry, et al., 2002)
In the pond water, aerobic photosynthetic microbes abound. These microbes include cyanobacteria and algae, which harvest light energy and release O2 as a by-product. With the energy from sunlight, the microbes fix CO2 and produce organic molecules. Algae and cyanobacteria appear quickly in the upper portion of the water column, where sunlight is abundant. By producing oxygen, these organisms help to keep this zone aerobic. This watery top layer contains an interesting diversity of microbes, green algae, cyanobacteria, various aerobic bacteria, fungi and protozoa. (Delia Castro Anderson and Rosalina V. Hairston 1999) The production of oxygen gas at the top of the column helps maintain the O2 gradient.
Each layer of

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