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RFLP involves unique combination of sequences inherited from the parents of an individual . So by using restriction enzymes to cut out a unique pattern we know each person has his/her own genetic pattern which can be used as a profile.
A restriction endonuclease “cuts” two DNA molecules at the same location. What can you assume is identical about the molecules at that location?
When the restriction endonuclease cuts two DNA molecules at the same location, then this indicates that the restriction sites are identical in the number of base pairs and the length of banded fragments.
Do any of your suspect samples appear to have EcoRI or Pstl recognition sites at the same location as the DNA from the crime scene? If so which suspects and what are the band lengths (bp) that …show more content…
Why is DNA sometimes refered to as a palindrome?
DNA is referred to palindromic because each one of its nucleotide sequences has complementary sequence which runs anti parallel which when reversed will read identical to the original sequence . For example the DNA sequence ACCTAGGT is palindromic because its complementary sequence reads TGGATCCA which when reversed in order will give us the former sequence.
Be sure to attach the printout of your gel photograph and standard curve to this report
Be sure to attach the printout of your gel photograph to this report.
Quantitative Analysis of DNA Fragment Size Data Table
DNA size marker
Band
Crime Scene
Suspect 1
Suspect 2
Suspect 3
Suspect 4
Suspect 5
Distance Actual Distance Approx. Distance Approx. Distance Approx. Distance Approx. Distance Approx. Distance Approx. (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp)
1
50
10,000
67
5100
71
4400
72
4200
66
5200
70
A restriction endonuclease “cuts” two DNA molecules at the same location. What can you assume is identical about the molecules at that location?
When the restriction endonuclease cuts two DNA molecules at the same location, then this indicates that the restriction sites are identical in the number of base pairs and the length of banded fragments.
Do any of your suspect samples appear to have EcoRI or Pstl recognition sites at the same location as the DNA from the crime scene? If so which suspects and what are the band lengths (bp) that …show more content…
Why is DNA sometimes refered to as a palindrome?
DNA is referred to palindromic because each one of its nucleotide sequences has complementary sequence which runs anti parallel which when reversed will read identical to the original sequence . For example the DNA sequence ACCTAGGT is palindromic because its complementary sequence reads TGGATCCA which when reversed in order will give us the former sequence.
Be sure to attach the printout of your gel photograph and standard curve to this report
Be sure to attach the printout of your gel photograph to this report.
Quantitative Analysis of DNA Fragment Size Data Table
DNA size marker
Band
Crime Scene
Suspect 1
Suspect 2
Suspect 3
Suspect 4
Suspect 5
Distance Actual Distance Approx. Distance Approx. Distance Approx. Distance Approx. Distance Approx. Distance Approx. (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp) (mm) size (bp)
1
50
10,000
67
5100
71
4400
72
4200
66
5200
70